Huh-7D 12 Cell Line human

1042712, human liver, Epithelial

• Sinónimos: Huh7 Cells, JTC-39 Cells, Japanese Tissue Culture-39 Cells
• Código UNSPSC: 41106514

Propiedades

• Nombre del producto: Huh-7D 12 Cell Line human, 01042712
• origen biológico: human liver
• modo de crecimiento: Adherent
• cariotipo: That of original Huh-7
• morfología: Epithelial
• productos: Not specified
• receptores: Not specified
• técnicas: cell culture | mammalian: suitable
• enfermedades relevantes: cancer
• temp. de almacenamiento: −196°C

Descripción

Descripción general

Huh-712D is one of a series of stable HDAg (Hepatitis Delta Antigen)-expressing human hepatoma cell lines that have been isolated and characterised. Huh-7D is a derivative of Huh-7 cells, which favor hepatitis C virus replication. Huh-7 cells are superior hepatic cell models compared to HepG2 cells for apolipoprotein based metabolic studies.

Origen línea celular

Human hepatocellular carcinoma

Descripción línea celular

Huh-712D is one of a series of stable HDAg (Hepatitis Delta Antigen)-expressing human hepatoma cell lines that have been isolated and characterised.

Aplicación

Study of intracellular traffic of delta virus antigens and RNA, viral RNA replication and early virus assembly events, and interaction between HDV and HBV.

Perfil del ADN

STR-PCR Data: Amelogenin: X
CSF1PO: 11
D13S317: 10
D16S539: 10
D5S818: 12
D7S820: 11
THO1: 7
TPOX: 8,11
vWA: 16,18

Medio de cultivo

DMEM (D6546) + 2mM L-Glutamine (G7513) + 10% FBS / FCS (F2442)

Rutina de subcultivo

Split sub-confluent cultures (70-80%) 1:3 to 1:5 i.e. seeding at 3-5x10,000 cells/cm² using 0.25% trypsin/EDTA; 5% CO₂; 37 C. Media change twice a week

Otras notas

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