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Merck

C2010

Sigma-Aldrich

Concanavalin A from Canavalia ensiformis (Jack bean)

Type IV, lyophilized powder

Sinónimos:

Con A

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About This Item

Número de CAS:
EC Number:
MDL number:
UNSPSC Code:
23151817
NACRES:
NA.32

type

Type IV

form

lyophilized powder

potency

<64 μg per mL agglutination activity

impurities

Carbohydrate, essentially free
salt, essentially free

storage temp.

−20°C

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General description

Concanavalin A (con A) is a plant lectin refined from jack beans. It is a tetrameric protein, which has four identical 28 kDa subunits. Con A has the ability to connect with the mannose residues of several glycoproteins and can stimulate lymphocytes.

Application

Concanavalin A from Canavalia ensiformis (Jack bean) has been used:
  • as T-lymphocyte mitogen in lymphocyte proliferation assay for correlations with organochlorine contaminants (OCs)
  • for in vitro stimulation of T cell hybridomas, BW5147 cells and primary CD4+ T cells for T cell vaccination and for induction of hepatitis
  • for the stimulation of mice splenocytes in the suppression of T-lymphocytes proliferation assay
  • for the stimulation of spleen in mitogen assays

Biochem/physiol Actions

Con A is not blood group specific but has an affinity for terminal α-D-mannosyl and α-D-glucosyl residues. Ca2+ and Mn2+ ions are required for activity. Con A dissociates into dimers at pH 5.6 or below. Between pH 5.8 and pH 7.0, Con A exists as a tetramer; above pH 7.0 higher aggregates are formed. Con A exhibits mitogenic activity which is dependent on its degree of aggregation. Succinylation results in an active dimeric form which remains a dimer above pH 5.6.

Quality

Highly purified by specific affinity chromatography

Analysis Note

Where reported, agglutination activity is expressed in μg/ml and is determined from serial dilutions in phosphate buffered saline, pH 6.8, containing Ca2+ and Mn2+ of a 1 mg per mL solution. This activity is the lowest concentration to agglutinate a 2% suspension of human erythrocytes after 1 hr incubation at 25 °C.

pictograms

Health hazard

signalword

Danger

Hazard Classifications

Repr. 2 - Resp. Sens. 1 - Skin Sens. 1

Storage Class

13 - Non Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Faceshields, Gloves


Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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