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B4882

Sigma-Aldrich

Bromelain from pineapple stem

≥3 units/mg protein

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About This Item

Número de CAS:
37189-34-7
Comisión internacional de enzimas:
3.4.22.32 (BRENDA, IUBMB)
EC Number:
253-387-5
MDL number:
MFCD00081423
UNSPSC Code:
12352204
NACRES:
NA.54

form

lyophilized powder

Quality Level

200

specific activity

≥3 units/mg protein

composition

Protein, ≥30% biuret

storage temp.

−20°C

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Application

Bromelain from pineapple stem has been used:
  • to evaluate the aqueous micellar two-phase systems composed of ionic liquids as co-surfactants
  • as a positive control in sodium dodecyl sulfate (SDS) / polyacrylamide gel electrophoresis (PAGE) and in cells proliferation assay
  • to study its potential analgesic effect and to evaluate its hydrolyzingeffects on proenkephalin (PENK)-derived peptides containing enkephalin sequences

Bromelain may be used to inhibit the biosysnthesis of proinflammatory prostaglandins. It may be used to reduce clotting efficiency. Bromelain, from pineapple stem, has been used to make enzymatic hydrolysates of honeybee-collected pollen .

Biochem/physiol Actions

Bromelain is a cysteine endopeptidase with broad specificity for cleavage of proteins. Bromelain may be from a stem or piece of fruit. Product B4882 is from pineapple stem. Product P3169 (pGlu-Phe-Leu-p-nitroanilide) may be used as a chromogenic substrate for the assay of bromelain.

Unit Definition

One unit will release 1.0 micromole of p-nitrophenol from N-alpha-CBZ-L-Lysine p-nitrophenyl ester per minute at pH 4.6 at 25 °C. One old titrimetric unit (pH 4.5 at 45 °C) is equivalent to approximately 1.7 new units (pH 4.6 at 25 °C).

Preparation Note

The product may be suspended in acetate buffer, pH 4.5 at 1 mg/mL concentration, yielding a hazy, off-white suspension.

inhibitor

Referencia del producto
Descripción
Precios

E3132

E-64, protease inhibitor

substrate

Referencia del producto
Descripción
Precios

P3169

pGlu-Phe-Leu p-nitroanilide, protease substrate

C3637

Z-L-Lys-ONp hydrochloride

pictograms

Health hazardExclamation mark
GHS08,GHS07

signalword

Danger

Hazard Classifications

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

target_organs

Respiratory system

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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In vitro antiproliferative activity of fresh pineapple juices on ovarian and colon cancer cell lines
Gani MBA, et al.
International Journal of Peptide Research and Therapeutics, 21(3), 353-364 (2015)
Enkephalin related peptides are released from jejunum wall by orally ingested bromelain
Orlandi-Mattos PE, et al.
Peptides, 115, 32-42 (2019)
Recovery of bromelain from pineapple stem residues using aqueous micellar two-phase systems with ionic liquids as co-surfactants
Vicente FA, et al.
Process. Biochem., 51(4), 528-534 (2016)
Soghra Khatun Haq et al.
European journal of biochemistry, 269(1), 47-52 (2002-01-11)
Equilibrium studies on the acid included denaturation of stem bromelain (EC 3.4.22.32) were performed by CD spectroscopy, fluorescence emission spectroscopy and binding of the hydrophobic dye, 1-anilino 8-naphthalene sulfonic acid (ANS). At pH 2.0, stem bromelain lacks a well defined
V Grabovac et al.
International journal of pharmaceutics, 326(1-2), 153-159 (2006-09-05)
The aim of this study was to investigate the influence of the proteolytic enzyme bromelain on the permeation of heparin across the gastrointestinal epithelial barrier. Stability of the complex and effect of heparin on the enzymatic activity of bromelain was
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Protocolos

HPLC Analysis of Bromelain on TSKgel® BioAssist® S vs. Competitive S Column

Protocol for HPLC Analysis of Bromelain on TSKgel® BioAssist® S vs. Competitive S Column

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