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B0519

Sigma-Aldrich

Biotin−Agarose

PBS suspension

Sinónimos:

Vitamin H Agarose

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About This Item

MDL number:
MFCD00163072
UNSPSC Code:
41106500
NACRES:
NA.56
En este momento no podemos mostrarle ni los precios ni la disponibilidad

form

PBS suspension

Quality Level

200

extent of labeling

≥30 mg avidin per mL packed resin
2-6 μmol per mL

matrix

4% beaded agarose

matrix activation

epoxy

matrix attachment

carboxy (amide linkage); resin remains relatively uncharged over wide pH range

matrix spacer

13 atoms

application(s)

life science and biopharma

storage temp.

2-8°C

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Application

Biotin contains a high affinity for binding to avidin or streptavidin. It has been widely used in biochemistry and molecular biology for detecting and labeling proteins.

Physical form

Suspension in phosphate buffered saline containing 0.02% sodium azide

Storage Class

10 - Combustible liquids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

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Certificados de análisis (COA)

Lot/Batch Number
0000393799
0000393800
0000393799
0000393800
0000292851

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A P Sibler et al.
Journal of immunological methods, 224(1-2), 129-140 (1999-06-05)
In vivo biotinylation of antibody fragments with a gene fusion approach is a realistic alternative to conventional in vitro chemical labelling. We have previously reported the construction of a vector system suitable for the bacterial expression of the binding fragment
Dietmar Eberbeck et al.
Journal of nanobiotechnology, 6, 4-4 (2008-03-13)
The binding reaction of the biomolecules streptavidin and anti-biotin antibody, both labelled by magnetic nanoparticles (MNP), to biotin coated on agarose beads, was quantified by magnetorelaxometry (MRX). Highly sensitive SQUID-based MRX revealed the immobilization of the MNP caused by the
P A Smith et al.
Nucleic acids research, 26(6), 1414-1420 (1998-04-29)
The high affinity binding interaction of biotin to avidin or streptavidin has been used widely in biochemistry and molecular biology, often in sensitive protein detection or protein capture applications. However, in vitro chemical techniques for protein biotinylation are not always
K J Airenne et al.
Gene, 144(1), 75-80 (1994-06-24)
A recombinant avidin (re-Avd), containing amino acids (aa) 1-123 of the native chicken egg-white Avd, was produced in Escherichia coli. When cells were grown at 37 degrees C production was over 1 microgram/ml, due to altering the codon preference of
Biotinylated CdSe/ZnSe nanocrystals for specific fluorescent labeling.
Charvet, N., et al.
Journal of Materials Chemistry, 14, 2638-2642 (2004)
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Questions

1–3 of 3 Questions  

  1. Salve ho acquistato questo prodotto. Esiste un protocollo di massima per il suo utilizzo?

    1 answer

    1. Unfortunately, a standard protocol is not available for this product. The sample binding capacity protocol below may be helpful:
      1. Pipette 2.0 mls of resin into pre-calibrated, fritted columns and allow the resin to pack. Measure the packed volume in milliliters.
      2. Wash the resin with 5 packed volumes of 0.5 M sodium chloride.
      3. Equilibrate the resin with 25 ml packed volumes of phosphate buffered saline (PBS), product P4417. Check the pH of effluent after the wash. The pH should be 6.75 to 7.25. If necessary, equilibrate with additional 25 X packed volume.
      4. Dissolve avidin from egg white, product A9275 at 80 mg protein/ml in PBS. Check the pH and record. If necessary, adjust the pH to 6.95 to 7.05 with 0.1 N HCl.
      5. Determination of Avidin load:
      PBS 10.0 ml
      Avidin at 80 mg/ml 0.05 ml
      Mix by inversion and measure A (280 nm) versus PBS.
      6. Calculation of Avidin Load:
      [(Net Absorbance (280 nm) x 10. 05]/(0.05 x 1.54)
      7. For each ml of packed gel in the column, add 1.0 ml of the avidin solution. Allow the column gel to equilibrate in the presence of the avidin for one hour with intermittent swirling.
      8. Wash the column with 100 packed volumes of PBS. Mix each wash by inversion and measure A (280nm) versus PBS.
      Calculation of wash:
      [(Net Absorbance (280nm) x 100] / (1.54)
      9. Bound avidin to biotin agarose= mg of avidin labeled - total avidin in wash (unbound).
      10. Binding capacity = (Bound avidin to biotin-agarose)/ml of packed gel.

      Additional references and general protocols are also available at the links provided below:
      https://www.sigmaaldrich.com/search/b0519?focus=papers&page=1&perpage=30&sort=relevance&term=B0519&type=citation_search
      https://www.sigmaaldrich.com/applications/protein-biology/protein-pulldown

      Helpful?

  2. Could you provide the protocol for this product?

    Could you provide the protocol for this product?

    1 answer

    1. Please refer to the following literature for guidance:
      - https://www.nature.com/articles/srep42849
      - https://aiche.onlinelibrary.wiley.com/doi/full/10.1002/btpr.2293

      Helpful?

  3. Is there a protocol available for this product?

    1 answer

    1. Unfortunately, there is no in-house protocol available for this product.

      Helpful?

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