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Merck

A4327

Sigma-Aldrich

Albúmina from human serum

lyophilized powder, essentially protease free, ≥96% (agarose gel electrophoresis)

Sinónimos:

HSA

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About This Item

Número de CAS:
Número CE:
Número MDL:
Código UNSPSC:
12352202
NACRES:
NA.25

origen biológico

human

Nivel de calidad

Ensayo

≥96% (agarose gel electrophoresis)

Formulario

lyophilized powder

mol peso

monomer calculated mol wt 66478 Da

técnicas

tissue culture: suitable

impurezas

HIV I and HIVII, HCV and HBsAg, tested negative

solubilidad

H2O: soluble 50 mg/mL

Nº de acceso UniProt

actividad extraña

Alkaline phosphatase ≤0.001 units/mg protein
Peroxidase ≤0.001 units/mg protein
Protease ≤0.0001 units/mg protein

temp. de almacenamiento

2-8°C

cadena SMILES

FC(F)(F)C(F)(F)C(F)(F)F

InChI

1S/C3F8/c4-1(5,2(6,7)8)3(9,10)11

Clave InChI

QYSGYZVSCZSLHT-UHFFFAOYSA-N

Información sobre el gen

human ... ALB(213)

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Descripción general

Albumin is the most copious protein in blood plasma. Produced in the liver, serum albumin serves as a carrier protein for biomolecules like as fatty acids, amino acids, and steroids.

This human serum albumin (HSA) product has been specially tested and evaluated to be low in the following properties:
  • Essentially protease-free / low protease content
  • Low alkaline phosphatase content
  • Low peroxidase content

Serum albumin is used in various applications related to molecular biology, notably as an inert protein stabilizer for stock solutions of enzymes and proteins of interest. In such applications, it is thus important for the HSA to be protease free, to maintain the integrity of the protein or enzyme of interest.

Protease-free HSA is especially useful for applications like:
  • Enzyme assays
  • Protein-based assays
  • Protease-sensitive techniques such as:
(a) Enzyme immunoassay (EIA)
(b) Nucleic acid hybridization
(c) Radioimmunoassay (RIA)

Aplicación

Albumin was used to test its effect on the in vitro bactericidal activity of cefditoren against penicillin-resistant Streptococcus pneumonia. It has been clinically used in serious and often life-threatening conditions, such as shock and blood loss due to trauma, burns, and surgery.
It was used to test the effect of non-enzymatic glycation on the unfolding of human serum albumin. It was also used to test the two distinct pathways used by intestinal epithelial cells for HLA class II antigen processing.

Acciones bioquímicas o fisiológicas

La seroalbúmina funciona como una proteína transportadora para esteroides, ácidos grasos y hormonas tiroideas, y es vital en la regulación de las presiones osmóticas coloidales de la sangre. También se ha observado que la albúmina se une a sustancias exógenas, en particular medicamentos (ibuprofeno, warfarina) y que influye fuertemente en su farmacocinética. El estrés oxidativo que induce cambios en el estado rédox de la albúmina tiene efectos muy variados sobre su función fisiológica.
Las albúminas son proteínas monómeras solubles que se encuentran en los líquidos y los tejidos de los animales y en algunas semillas vegetales. La seroalbúmina funciona como proteína transportadora para esteroides, ácidos grasos y hormonas tiroideas. Las seroalbúminas son también vitales en la regulación de las presiones osmóticas coloidales de la sangre.

Características y beneficios

  • Low protease activity / essentially protease-free (≤ 0.0001 units/mg protein)
  • Low alkaline phosphatase activity (≤ 0.001 units/mg protein)
  • Low peroxidase activity (≤ 0.001 units/mg protein)

Nota de preparación

Prepared from A1653

Otras notas

Encontrará más información en seroalbúmina humana.

Cláusula de descargo de responsabilidad

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

Código de clase de almacenamiento

10 - Combustible liquids

Clase de riesgo para el agua (WGK)

WGK 3

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable

Equipo de protección personal

Eyeshields, Gloves


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