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50649

Sigma-Aldrich

Glyoxal solution

for molecular biology, BioReagent, ~40% in H2O (~8.8 M)

Sinónimos:

Ethanedial, Oxalaldehyde

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About This Item

Fórmula lineal:
OHCCHO
Número de CAS:
107-22-2
Peso molecular:
58.04
Beilstein:
1732463
Número MDL:
MFCD00006957
Código UNSPSC:
12352114
ID de la sustancia en PubChem:
57651264
NACRES:
NA.52
En este momento no podemos mostrarle ni los precios ni la disponibilidad

grado

for molecular biology

Nivel de calidad

100

Línea del producto

BioReagent

Formulario

liquid

concentración

~40% in H2O (~8.8 M)

impurezas

DNases, none detected
 RNases, none detected
 phosphatases, none detected
 proteases, none detected

índice de refracción

n20/D 1.409

trazas de anión

chloride (Cl-): ≤100 mg/kg
sulfate (SO42-): ≤100 mg/kg

trazas de catión

Ca: ≤5 mg/kg
Cd: ≤5 mg/kg
Co: ≤5 mg/kg
Cr: ≤5 mg/kg
Cu: ≤5 mg/kg
Fe: ≤5 mg/kg
K: ≤5 mg/kg
Mg: ≤5 mg/kg
Mn: ≤5 mg/kg
Na: ≤300 mg/kg
Ni: ≤5 mg/kg
Pb: ≤5 mg/kg
Zn: ≤5 mg/kg

temp. de almacenamiento

2-8°C

cadena SMILES

[H]C(=O)C([H])=O

InChI

1S/C2H2O2/c3-1-2-4/h1-2H

Clave InChI

LEQAOMBKQFMDFZ-UHFFFAOYSA-N

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Descripción general

Glyoxal is a nucleic acid denaturant. It acts as a specific probe for homopurine strands of DNA.

Aplicación

Suitable for
  • nucleic acid denaturation
  • binding of DNA/RNA to nitrocellulose membrane

Otras notas

This form of glyoxal is composed of 3 moles of glyoxal and 2 moles of water in a relatively stable configuration.
May precipitate on storage; redissolve at 50-60 °C.

Precaución

May precipitate on storage; redissolve at 50-60 °C.

Principio

Glyoxal forms stable complex with guanine residues that results in denaturation of nucleic acids.

Producto relacionado

Referencia del producto
Descripción
Precios

Pictogramas

Health hazardExclamation mark
GHS08,GHS07

Palabra de señalización

Warning

Clasificaciones de peligro

Acute Tox. 4 Inhalation - Eye Irrit. 2 - Muta. 2 - Skin Irrit. 2 - Skin Sens. 1 - STOT SE 3

Órganos de actuación

Respiratory system

Código de clase de almacenamiento

10 - Combustible liquids

Clase de riesgo para el agua (WGK)

WGK 1

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable

Equipo de protección personal

Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter

Elija entre una de las versiones más recientes:

Certificados de análisis (COA)

Lot/Batch Number
BCBL7249V
394949/1
367628/1
354841/1
70586/1

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Si necesita una versión concreta, puede buscar un certificado específico por el número de lote.

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Hybridization of denatured RNA transferred or dotted nitrocellulose paper.
P S Thomas
Methods in enzymology, 100, 255-266 (1983-01-01)
R N Skopp et al.
Analytical biochemistry, 169(1), 132-137 (1988-02-15)
This paper describes agarose gel electrophoresis and silver staining of denatured RNAs. Glyoxal- or formaldehyde-denatured RNAs are electrophoresed in an agarose gel cast on a plastic support using an inert, low conductivity buffer. Following electrophoresis, the gel is stained with
M Vojtisková et al.
Journal of biomolecular structure & dynamics, 5(2), 283-296 (1987-10-01)
Plasmid pEJ4, which is a derivative of pUC19 containing an insert with 60-bp-long homopurine.homopyrimidine tract from sea urchin P. miliaris histone gene spacer, was studied by chemical probes of the DNA structure osmium tetroxide and glyoxal. The former probe reacts
Niklas Bosaeus et al.
Nucleic acids research, 42(12), 8083-8091 (2014-05-20)
Overstretching of DNA occurs at about 60-70 pN when a torsionally unconstrained double-stranded DNA molecule is stretched by its ends. During the transition, the contour length increases by up to 70% without complete strand dissociation. Three mechanisms are thought to
Sabrina Gensberger et al.
Analytical and bioanalytical chemistry, 403(10), 2923-2931 (2012-03-03)
High-fructose corn syrup (HFCS) is a widely used liquid sweetener produced from corn starch by hydrolysis and partial isomerization of glucose to fructose. During these processing steps, sugars can be considerably degraded, leading, for example, to the formation of reactive
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Questions

1–3 of 3 Questions  

  1. How can I determine the shelf life / expiration / retest date of this product?

    1 answer

    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdf

      Helpful?

  2. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer

    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

      Helpful?

  3. Is this glyoxal deionized?

    1 answer

    1. This glyoxal is not deionized. As mentioned in the DESCRIPTION section under 'Other Notes', the form of glyoxal is composed of 3 moles of glyoxal and 2 moles of water in a relatively stable configuration.

      Helpful?

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