NIST2373
Genomic DNA Standards for HER2 Measurements
NIST®SRM®
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About This Item
UNSPSC Code:
41116107
NACRES:
NA.24
Productos recomendados
form
liquid
manufacturer/tradename
NIST®
technique(s)
PCR: suitable
qPCR: suitable
application(s)
genomic analysis
storage temp.
2-8°C
General description
Genomic DNA Standards are standard reference materials (SRM 2373) containing genomic DNA extracted from five breast cancer cell lines with different amounts of amplification of the HER2 gene. The five components are genomic DNA materials derived from human cell lines SK-BR-3, MDA-MB-231, MDA-MB-361, MDA-MB-453, and BT-474, labeled A, B, C, D, and E, respectively. A unit of SRM 2373 consists of five vials, one of each component, containing approximately 100 μL of DNA solution.
SRM_2373_cert
SRM_2373_SDS
SRM_2373_cert
SRM_2373_SDS
Application
Genomic DNA Standards for HER2 Measurements is intended for use in the assignment of ratios of the human epidermal growth factor receptor 2 gene (HER2, the official gene symbol ERBB2) to unamplified reference genes. It is suitable for use with quantitative polymerase chain reaction (qPCR) and digital polymerase chain reaction (dPCR) assays.
Features and Benefits
The use of this material will provide confidence in the assignment of certified ratios to the secondary standard DNA solutions for routine use with qPCR and dPCR assays.
Other Notes
- SRM 2373 is intended for research use. This is a human source material.
- Expiration details, storage, and usage instructions are provided on the NIST certificate
- Notes on biomaterial handling, disposal, and transport documented in MSDS
Legal Information
NIST is a registered trademark of National Institute of Standards and Technology
SRM is a registered trademark of National Institute of Standards and Technology
Storage Class
12 - Non Combustible Liquids
wgk_germany
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
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Hua-Jun He et al.
Biomolecular detection and quantification, 8, 1-8 (2016-06-24)
NIST standard reference material (SRM) 2373 was developed to improve the measurements of the HER2 gene amplification in DNA samples. SRM 2373 consists of genomic DNA extracted from five breast cancer cell lines with different amounts of amplification of the
Hua-Jun He et al.
Clinical chemistry and laboratory medicine, 57(8), 1142-1152 (2019-05-22)
Background The National Institute of Standards and Technology (NIST) Reference Material RM 8366 was developed to improve the quality of gene copy measurements of EGFR (epidermal growth factor receptor) and MET (proto-oncogene, receptor tyrosine kinase), important targets for cancer diagnostics
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