71017BC

Assurance^® GDS Top 7 STEC (eae) Tq Kit

suitable for PCR testing in Food and Beverages applications

• UNSPSC Code: 41106302
• NACRES: NA.85

Propiedades

• product name: GDS Top 7 STEC (eae) Tq, BioControl, Molecular based PCR test for the detection of the eae gene in "Top Seven" Shiga Toxigenic E. coli (STEC) in food
• agency: AOAC^® (PTM)
• Quality Level: 200
• shelf life: 6‑12 mo.
• packaging: pkg of 100 tests
• manufacturer/tradename: Assurance GDS^®; BioControl Systems 71017-100
• technique(s): PCR: suitable
• application(s): food and beverages; pathogen testing
• compatibility: for use with GDS Rotor-Gene
• storage temp.: 2-8°C
• suitability: enterovirulent E. coli (EEC) (shiga toxigenic)

Descripción

General description

Assurance^® GDS is an advanced genetic detection system for food pathogens. The Top 7 STEC (eae) Tq is an automated nucleic acid amplification system for the detection of the eae gene in the following Top 7 O serogroups of E. coli: O103, O111, O121, O145, O26, O45, and O157. Assurance^® GDS utilizes a proprietary PickPen^® IMS-based sample preparation procedure to quickly and effectively capture organisms belonging to the 7 specific Top STEC O serogroups prior to genetic analysis for the presence of the eae gene.

Application

Assurance^® GDS Top 7 STEC (eae) Tq is designed for use in conjunction with Assurance^® GDS Shiga Toxin Genes (Top 7) Tq to detect the presence of Shiga Toxigenic E. coli (STEC) in foods, ingredients, and environmental samples.

Features and Benefits

• Sequential approach to detecting Top STEC. Following sample preparation with IMS against the Top 7 O groups, samples are analyzed for the presence of (eae) with the GDS Top 7 STEC (eae) assay. Positive samples are subsequently analyzed for Shiga Toxin genes with the Assurance^® GDS Shiga Toxin Genes Top 7 assay.
• Top 7 STEC (includes E. coli O157:H7)
• Single-step 10-18 h enrichment in mEHEC, enrichment media common to all BioControl methods for E.coli O157:H7, Shiga Toxin genes, and Top STEC

Components

• Top STEC (eae) Amplification Tubes Tq
• Top 7 STEC Concentration Reagent
• Resuspension Buffer Tq
• Top STEC Wash Solution

Compatibility

BioControl GDS

Testing Method

PCR, Immunomagnetic Separation (IMS)

Legal Information

AOAC is a registered trademark of AOAC International

Assurance is a registered trademark of Merck KGaA, Darmstadt, Germany

Assurance GDS is a registered trademark of BioControl Systems, Inc.

PICKPEN is a registered trademark of Merck KGaA, Darmstadt, Germany

Información de seguridad

• Pictograms: GHS05
• signalword: Warning
• hcodes: H290
• pcodes: P234 - P390
• Hazard Classifications: Met. Corr. 1
• Storage Class: 8A - Combustible corrosive hazardous materials