12041677001

CDP-Star^®, ready-to-use

>98%, solution, suitable for dot blot, suitable for Northern blotting, suitable for Southern blotting

• UNSPSC Code: 12352204

Propiedades

• description: Disodium 2-chloro-5-(4-methoxyspiro {1,2-dioxetane-3,2′-(5′-chloro)tricyclo[3.3.1.13,7 ]decan}-4-yl)-1-phenyl phosphate
• Quality Level: 100
• assay: >98%
• form: solution
• packaging: bottle of 2 × 50 mL ([0.25 mM])
• manufacturer/tradename: Roche
• technique(s): Northern blotting: suitable; Southern blotting: suitable; dot blot: suitable
• storage temp.: 2-8°C

Descripción

Publishing

Purity: Purified by HPLC.

Application

Used for the nonradioactive detection of labeled nucleic acid probes on membrane blots:

• Southern blot
• Northern blot
• Dot blot
• Colony and plaque hybridization
• Gel shift assays

Used for the nonradioactive detection of labeled nucleic acid probes on membrane blots:

• Southern blot
• Northern blot
• Dot blot
• Colony and plaque hybridization
• Gel shift assays
• Chemiluminescent detection.

Features and Benefits

Contents
0.25 mM solution (0.124 mg/ml), ready-to-use, colorless solution

CDP-Star is a chemiluminescent substrate for alkaline phosphatase that enables fast, ultra-sensitive detection of biomolecules by producing extremely rapid visible light. Light emission is recorded on X-ray film or on luminescence imager systems.

• Save time with a fast, ultra-sensitive substrate.
• Easily strip and reprobe membranes.
• Take multiple exposures of up to two days for each experiment.

Reuse the substrate by filtering and storing in sodium azide.

Preparation Note

Storage conditions (working solution): Washing Buffer: 15 to 25 °C, stable
Maleic Acid Buffer: 15 to 25 °C, stable
Detection Buffer: 15 to 25 °C, stable
Blocking Solution: always prepare freshly
Antibody Solution: 2 to 8 °C

Other Notes

• First exposure to X-ray film should be 5-15 minutes. Examine film and then determine other exposure times based on initial result.
• No preincubation step required.
• Apply substrate quickly to avoid gray shadows from substrate drops and drying of membrane (leads to uneven, high background).
• Do not use plastic wrap to cover blot; use hybridization bags, acetate sheet protectors, or two sheets of transparent film.
• Exposure times are shorter than with CSPD.
• Do not use nitrocellulose membranes.

Repeat exposures can be made up to two days after the addition of substrate.

For life science research only. Not for use in diagnostic procedures.

Legal Information

CDP-Star is a registered trademark of Tropix, Inc.

Información de seguridad

• Storage Class: 12 - Non Combustible Liquids
• wgk_germany: nwg
• flash_point_f: does not flash
• flash_point_c: does not flash