MABE1130

Anti-Hsp70/Hsp72 Antibody, clone 2H9

clone 2H9, from mouse

• Sinónimos: Heat shock 70 kDa protein 1A, Heat shock 70 kDa protein 1, HSP70.1
• Código UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Propiedades

• origen biológico: mouse
• Nivel de calidad: 100
• forma del anticuerpo: purified immunoglobulin
• tipo de anticuerpo: primary antibodies
• clon: 2H9, monoclonal
• reactividad de especies: bovine, human
• técnicas: western blot: suitable
• isotipo: IgG1κ
• Nº de acceso NCBI: NP_776975.1
• Nº de acceso UniProt: Q27975
• Condiciones de envío: wet ice
• modificación del objetivo postraduccional: unmodified
• Información sobre el gen: human ... HSPA1A(3303)

Descripción

Descripción general

Heat shock 70 kDa protein 1A (UniProt Q27975; also known as Heat shock 70 kDa protein 1, HSP70.1) is encoded by the HSPA1A (also known as HSP70-1) gene (Gene ID 281825 & 282254) in bovine species. The mammalian HSP70 family consists of the mitochondrial HSP75, the endoplasmic HSP78, the cytoplasmic HSC70 (a.k.a. HSC73 and HSP73) and its inducible counterpart HSP72 (a.k.a. HSP70). The latter two have similar structures and properties, and both of them bind misfolded, newly synthesized polypeptides in an ATP-dependent manner, reflecting their chaperonic activity. All cells constitutively express HSC70. On the other hand, the expression of HSP72 in normal cells is mainly stress inducible, although constitutively high HSP72 expressions are observed in malignant human tumors of various origins. The induced HSP72 expression enables stressed cells to better cope with the elevation of unfolded/denatured proteins and to prevent apoptosis under various stress conditions.

Especificidad

Clone 2H9 reacts with the heat schock-inducible HSP72 (HSPA1A), but not the constitutively expressed HSPA8/Hsc70 (HSC73) (Demidov, O.N., et al. (1999). Eur. J. Cardiothorac. Surg. 16(4):444-449). Reactivity toward HSPA1B and HSPA2 (UniProt Q27965 and P34933) has not been determined.

Inmunógeno

Bovine skeletal muscle Hsp70/Hsp72.

Aplicación

Anti-Hsp70/Hsp72 Antibody, clone 2H9 is an antibody against Hsp70/Hsp72 for use in Western Blotting.

Immunoprecipitation Analysis: Clone 2H9 ascites fluid immunoprecipitated Hsp70/Hsp72 from human U-937 myeloid leukemia cell lysates (Courtesy of Dr. Irina V. Guzhova and Dr. Boris Margulis, Russian Academy of Sciences).
Western Blotting Analysis: A representative lot detected Hsp70/Hsp72 expression and siRNA-mediated Hsp70 down-regulation in HeLa cells (Rohde, M., et al. (2005). Genes Dev. 19(5):570-582).
Western Blotting Analysis: A representative lot detected the heat schock-inducible Hsp70/Hsp72, but not the constitutively expressed HSPA8/Hsc70 (HSC73) in heat shock protein preparations from bovine skeletal muscle and human myocardium tissue (Demidov, O.N., et al. (1999). Eur. J. Cardiothorac. Surg. 16(4):444-449).
Western Blotting Analysis: A representative lot detected heat shock-induced Hsp70 expression in four human leukemia cell lines (U937, Jurkat, Molt4, HL60), but not in murine myeloma NSO cells (Lasunskaia, E.B., et al. (1997). Apoptosis. 2(2):156-163).

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Chaperones

Calidad

Evaluated by Western Blotting in MCF7 cell lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected Hsp70/Hsp72 in 10 µg of MCF7 cell lysate.

Descripción de destino

~70 kDa observed.

Forma física

Format: Purified

Protein G Purified

Purified mouse monoclonal IgG1κ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Almacenamiento y estabilidad

Stable for 1 year at 2-8°C from date of receipt.

Otras notas

Concentration: Please refer to lot specific datasheet.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Código de clase de almacenamiento: 12 - Non Combustible Liquids
• Clase de riesgo para el agua (WGK): WGK 1
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable