MAB326
Anti-CNPase Antibody, clone 11-5B
clone 11-5B, Chemicon®, from mouse
Sinónimos:
2′3′-Cyclic Nucleotide 3′-Phosphohydrolase
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About This Item
Código UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Productos recomendados
origen biológico
mouse
Nivel de calidad
forma del anticuerpo
purified immunoglobulin
tipo de anticuerpo
primary antibodies
clon
11-5B, monoclonal
reactividad de especies
canine, sheep, mouse, pig, bovine, rat, rabbit, human
no debe reaccionar con
guinea pig, chicken
fabricante / nombre comercial
Chemicon®
técnicas
immunocytochemistry: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
western blot: suitable
isotipo
IgG1
Nº de acceso NCBI
Nº de acceso UniProt
Condiciones de envío
wet ice
modificación del objetivo postraduccional
unmodified
Información sobre el gen
human ... CNP(1267)
Descripción general
CNPase (2′, 3′-cyclic nucleotide 3′-phosphodiesterase [or -phosphohydrolase], EC 3.1.4.37) is present in very high levels in brain and peripheral nerve. This enzyme is found almost exclusively in oligo-dendrocytes and Schwann cells, the cells that form myelin in the central and peripheral nervous system, respectively.
Immunohistochemical localization of CNPase has shown the enzyme to be restricted to oligodendrocytes and Schwann cells. The enzyme appears to be distributed in single and double loose wraps of myelin and not in compact myelin as earlier thought by most investigators. CNPase is located on the inner and outer loops of myelin, paranodally and near the inner surface of the oligodendrocyte membrane. In plaque regions of multiple sclerosis patients, the enzyme is reduced, and when CNS myelin is decreased, CNPase is one of the earlier proteins to be lost from the myelin. In addition, an enzyme that is probably identical to brain CNPase is located in the outer rod segments within the visual system, and this protein is also recognized by the monoclonal antibody 11-5B. In mixed human gliomas, the enzyme levels are reduced, although about 5% of the oligodendrocytes occasionally show normal positive staining.
Immunohistochemical localization of CNPase has shown the enzyme to be restricted to oligodendrocytes and Schwann cells. The enzyme appears to be distributed in single and double loose wraps of myelin and not in compact myelin as earlier thought by most investigators. CNPase is located on the inner and outer loops of myelin, paranodally and near the inner surface of the oligodendrocyte membrane. In plaque regions of multiple sclerosis patients, the enzyme is reduced, and when CNS myelin is decreased, CNPase is one of the earlier proteins to be lost from the myelin. In addition, an enzyme that is probably identical to brain CNPase is located in the outer rod segments within the visual system, and this protein is also recognized by the monoclonal antibody 11-5B. In mixed human gliomas, the enzyme levels are reduced, although about 5% of the oligodendrocytes occasionally show normal positive staining.
Especificidad
CNPase, 48 and 46 kD polypeptides by SDS-PAGE. Differentiates clearly oligodendrocytes and Schwann cells from neurons, astrocytes, etc.
Inmunógeno
Purified human brain CNPase
Aplicación
Detect CNPase using this Anti-CNPase Antibody, clone 11-5B validated for use in IC, IH, IH(P) & WB.
Immunocytochemistry:
A previous lot was used on primary oligodendrocyte cultures.
Immunohistochemistry:
A previous lot was used on rat hippocampus tissue and rat spinal cord.
Immunoblotting of myelin, the Wolfgram protein fraction, the SN4 fraction, tissue sections and mixed glial tumors (oligodendrogliomas, etc.) CNPase I (46 kDa) and CNPase II (48 kDa), which are differentially regulated during development, with the larger protein being expressed earlier than CNPase I during development.
Immunohistochemistry on both fresh frozen and paraffin embedded tissue (microwave pretreatment, ctirate pH 6.0).
Immunoblot:
Immunoblotting of myelin, the Wolfgram protein fraction, the SN4 fraction, tissue sections
and mixed glial tumors (oligodendrogliomas, etc.)
Optimal working dilutions must be determined by end user.
A previous lot was used on primary oligodendrocyte cultures.
Immunohistochemistry:
A previous lot was used on rat hippocampus tissue and rat spinal cord.
Immunoblotting of myelin, the Wolfgram protein fraction, the SN4 fraction, tissue sections and mixed glial tumors (oligodendrogliomas, etc.) CNPase I (46 kDa) and CNPase II (48 kDa), which are differentially regulated during development, with the larger protein being expressed earlier than CNPase I during development.
Immunohistochemistry on both fresh frozen and paraffin embedded tissue (microwave pretreatment, ctirate pH 6.0).
Immunoblot:
Immunoblotting of myelin, the Wolfgram protein fraction, the SN4 fraction, tissue sections
and mixed glial tumors (oligodendrogliomas, etc.)
Optimal working dilutions must be determined by end user.
Research Category
Neuroscience
Neuroscience
Research Sub Category
Neuronal & Glial Markers
Neuronal & Glial Markers
Calidad
Routinely evaluated by Western Blot on Mouse Brain lysates.
Western Blot Analysis:
1:1000 dilution of this lot detected CNPASE on 10 μg of Mouse Brain lysates.
Western Blot Analysis:
1:1000 dilution of this lot detected CNPASE on 10 μg of Mouse Brain lysates.
Descripción de destino
48 & 46 kDa
Forma física
Format: Purified
Protein A purified
Purified mouse monoclonal IgG1 in buffer containing 0.02M phosphate buffer, 0.25 M NaCl with 0.1% sodium azide
Almacenamiento y estabilidad
Stable for 1 year at 2-8ºC from date of receipt.
Nota de análisis
Control
Western Blot: Fresh bovine whole brain extract, mouse brain lysate.
Immunohistochemistry: Rat hippocampus tissue, rat spinal cord tissue.
Western Blot: Fresh bovine whole brain extract, mouse brain lysate.
Immunohistochemistry: Rat hippocampus tissue, rat spinal cord tissue.
Otras notas
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Información legal
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Cláusula de descargo de responsabilidad
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Opcional
Referencia del producto
Descripción
Precios
Código de clase de almacenamiento
10 - Combustible liquids
Clase de riesgo para el agua (WGK)
WGK 2
Certificados de análisis (COA)
Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»
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