MAB13424
Anti-MMP-13 Antibody, clone VIIIA2
clone VIIIA2, Chemicon®, from mouse
Sinónimos:
Collagenase-3
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About This Item
Código UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Productos recomendados
origen biológico
mouse
Nivel de calidad
forma del anticuerpo
purified antibody
tipo de anticuerpo
primary antibodies
clon
VIIIA2, monoclonal
reactividad de especies
mouse, human, rabbit
fabricante / nombre comercial
Chemicon®
técnicas
immunohistochemistry: suitable
isotipo
IgG1
Nº de acceso NCBI
Nº de acceso UniProt
Condiciones de envío
wet ice
modificación del objetivo postraduccional
unmodified
Información sobre el gen
human ... MMP13(4322)
Especificidad
It recognizes proteins of ~60 kDa and ~48 kDa which are identified as pro (latent) and activate forms of matrix metalloproteinase-13 (MMP-13; also known as Collagenase-3). Shows no cross reactivity with the pro and active forms of other MMPs. Human collagenase-3 (MMP13) is a recently identified member of the matrix metalloproteinase (MMP) family that is expressed in breast carcinomas and in articular cartilage from arthritic patients. The MMP-13 gene has been isolated and characterized. This gene is composed of 10 exons and 9 introns and spans over 12.5 kb. The overall organization of the collagenase-3 gene is similar to that of other MMP genes clustered at chromosome 11q22, including fibroblast collagenase (MMP-1), matrilysin (MMP-7), and macrophage metalloelastase (MMP-12), but is more distantly related to genes coding for stromelysin-3 (MMP-11), gelatinase-A (MMP-2), and gelatinase-B (MMP-9), which map outside of this gene cluster. Cellular Localization: Cytoplasmic
Inmunógeno
Human recombinant collagenase-3 protein.
Aplicación
Immunohistochemistry (frozen and formalin-fixed/paraffin*): 2-4 μg/mL for 60 minutes at room temperature.
* Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at room temperature for 20 minutes.
Immunoprecipation: use protein G (2 μg/mg protein lysate)
Optimal working dilutions must be determined by end user.
* Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at room temperature for 20 minutes.
Immunoprecipation: use protein G (2 μg/mg protein lysate)
Optimal working dilutions must be determined by end user.
Research Category
Cell Structure
Cell Structure
Research Sub Category
MMPs & TIMPs
MMPs & TIMPs
This Anti-MMP-13 Antibody, clone VIIIA2 is validated for use in IH for the detection of MMP-13.
Forma física
Format: Purified
Liquid in 10 mM PBS, pH 7.4, with 0.2% BSA and 15 mM sodium azide.
Almacenamiento y estabilidad
Maintain at 2-8°C in undiluted aliquots for up to 12 months.
Nota de análisis
Control
POSITIVE CONTROL: Conditioned, serum-free medium from (PMA-treated) human fibrosarcoma HT-1080 cells. Bladder, breast, ovarian carcinomas.
POSITIVE CONTROL: Conditioned, serum-free medium from (PMA-treated) human fibrosarcoma HT-1080 cells. Bladder, breast, ovarian carcinomas.
Otras notas
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Información legal
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Cláusula de descargo de responsabilidad
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Código de clase de almacenamiento
12 - Non Combustible Liquids
Clase de riesgo para el agua (WGK)
WGK 2
Punto de inflamabilidad (°F)
Not applicable
Punto de inflamabilidad (°C)
Not applicable
Certificados de análisis (COA)
Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»
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Bu-Nam Jeon et al.
FEBS letters, 593(18), 2665-2674 (2019-06-22)
Dysregulated matrix metalloproteinase (MMP) gene expression is a major cause of the degradation of lung tissue that is integral to emphysema pathogenesis. Cigarette smoking (CS) increases MMP gene expression, a major contributor to emphysema development. We previously reported that Zbtb7c
Ann M Kennedy et al.
The Journal of clinical investigation, 115(10), 2832-2842 (2005-09-17)
MMPs, which degrade components of the ECM, have roles in embryonic development, tissue repair, cancer, arthritis, and cardiovascular disease. We show that a missense mutation of MMP13 causes the Missouri type of human spondyloepimetaphyseal dysplasia (SEMD(MO)), an autosomal dominant disorder
Kaige Ma et al.
Science advances, 10(7), eadi5501-eadi5501 (2024-02-14)
Osteoarthritis (OA) is characterized by cartilage damage, inflammation, and pain. Vascular endothelial growth factor receptors (VEGFRs) have been associated with OA severity, suggesting that inhibitors targeting these receptors alleviate pain (via VEGFR1) or cartilage degeneration (via VEGFR2). We have developed
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Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research, 37(5), 983-996 (2022-02-28)
Enchondromas and chondrosarcomas are common cartilage neoplasms that are either benign or malignant, respectively. The majority of these tumors harbor mutations in either IDH1 or IDH2. Glutamine metabolism has been implicated as a critical regulator of tumors with IDH mutations.
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International journal of biological sciences, 19(2), 675-690 (2023-01-13)
Pain is the major reason that patients suffering from osteoarthritis (OA) seek medical care. We found that vascular endothelial growth factors (VEGFs) mediate signaling in OA pain pathways. To determine the specific contributions of VEGFs and their receptors (VEGFRs) to
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