ABE203

Anti-JARID1D Antibody

from rabbit, purified by affinity chromatography

• Sinónimos: Lysine-specific demethylase 5D, Histocompatibility Y antigen, H-Y, Histone demethylase JARID1D, Jumonji/ARID domain-containing protein 1D, Protein SmcY
• Código UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Propiedades

• origen biológico: rabbit
• Nivel de calidad: 100
• forma del anticuerpo: affinity isolated antibody
• tipo de anticuerpo: primary antibodies
• clon: polyclonal
• purificado por: affinity chromatography
• reactividad de especies: mouse, human
• técnicas: immunocytochemistry: suitable; immunoprecipitation (IP): suitable; western blot: suitable
• Nº de acceso NCBI: NP_004644.2
• Nº de acceso UniProt: Q9BY66
• Condiciones de envío: wet ice
• modificación del objetivo postraduccional: unmodified
• Información sobre el gen: human ... KDM5D(8284)

Descripción

Descripción general

Also known as Lysine-specific demethylase 5D, this histone demethylase is specific for ′Lys-4′ of histone H3 and is known to play a central role in histone function and spermatogenesis. It demethylates trimethylated and dimethylated, but not monomethylated H3 ′Lys-4′. Uniprot.org states two different isoforms of this enzyme.

Inmunógeno

KLH-conjugated linear peptide corresponding to human JARID1D.

Aplicación

Immunocytochemistry Analysis: 2 µg/mL from a representative lot detected JARID1D in mESCs.

Immunoprecipitation Analysis: A representative lot immunoprecipitated JARID1D in human testis tissue lysate.

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Histone Modifying Proteins

Use Anti-JARID1D Antibody (rabbit polyclonal antibody) validated in WB, ICC, IP to detect JARID1D also known as Lysine-specific demethylase 5D, Histocompatibility Y antigen, H-Y, Histone demethylase JARID1D.

Calidad

Evaluated by Western Blot in human testis tissue lysate.

Western Blot Analysis: 0.5 µg/mL of this antibody detected JARID1D in 10 µg of human testis tissue lysate.

Descripción de destino

~174 kDa observed. Two isoforms may be observed due to alternative splicing at a molecular weight of ~167 kDa and ~174 kDa in some cell lysates. An uncharacterized band at a molecular weight of ~255 kDa may be observed in some cell lysates.

Forma física

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Almacenamiento y estabilidad

Stable for 1 year at 2-8°C from date of receipt.

Nota de análisis

Control
Human testis tissue lysate.

Otras notas

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Código de clase de almacenamiento: 12 - Non Combustible Liquids
• Clase de riesgo para el agua (WGK): WGK 1
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable