AB5519

Anti-Prodynorphin Antibody

Chemicon^®, from guinea pig

• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Propiedades

• biological source: guinea pig
• Quality Level: 100
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• purified by: affinity chromatography
• species reactivity: rat
• manufacturer/tradename: Chemicon^®
• technique(s): immunohistochemistry: suitable
• NCBI accession no.: NM_024411.2
• UniProt accession no.: P01213
• shipped in: dry ice
• target post-translational modification: unmodified
• Gene Information: human ... PDYN(5173)

Descripción

Specificity

ProDynorphin, rat.

Immunogen

Synthetic peptide from rat ProDynorphin.

Application

Anti-Prodynorphin Antibody is an antibody against Prodynorphin for use in IH.

Immunohistochemistry: 1:500

Optimal working dilutions must be determined by the end user.

APPLICATION NOTES FOR AB5519

IMMUNOHISTOCHEMISTRY

Perfusion: 1) Ca2+-free Tyrode′s solution followed by 2) paraformaldehyde-picric acid fixative and 3) 10% sucrose in PBS as a cryo-protectant. Tissues were rapidly dissected out and stored overnight in 0.1 M phosphate buffer (pH 7.4) containing 10% sucrose.

Slide-mounted tissue sections were incubated with blocking buffer for 1 hour at room temperature. Primary antibody was diluted in blocking buffer to the appropriate working dilution. Blocking buffer was removed and the slides were then incubated at 2-8°C for 18-24 hours with AB5519. After rinsing in PBS 3 times sections were incubated for 60 minutes at room temperature with Cy3-conjugated secondary antibodies. After mounting in a mixture of PBS and glycerol (1:3) containing 0.1% p-phenylenediamine, sections were examined with a Nikon Microphot-SA epifluorescence microscope.

Physical form

Affinity purified Guinea pig antibody in PBS containing 0.05% sodium azide.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Información de seguridad

• Storage Class: 10 - Combustible liquids
• wgk_germany: WGK 2
• flash_point_f: Not applicable
• flash_point_c: Not applicable