Saltar al contenido
Merck
Todas las fotos(1)

Key Documents

AB3046

Sigma-Aldrich

Anti-Uncoupling Protein 3 Antibody

Chemicon®, from rabbit

Sinónimos:

UCP3

Iniciar sesiónpara Ver la Fijación de precios por contrato y de la organización


About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

antibody form

affinity purified immunoglobulin

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

human, rat

manufacturer/tradename

Chemicon®

technique(s)

ELISA: suitable
immunohistochemistry: suitable
western blot: suitable

NCBI accession no.

UniProt accession no.

shipped in

dry ice

target post-translational modification

unmodified

Gene Information

human ... UCP3(7352)
rat ... Ucp3(25708)

General description

Human UCP3 long form (UCP3L) is a 312 aa mitochondrial uncoupling protein. It is only 57% and 73% homologous with human UCP1 and UCP2 respectively. Like other UCPs, UCP3 is predicted to contain 6 transmembrane domains. The UCP3S lacks the 6th transmembrane domain and is only 275 aa long. UCP3 has preferential expression in muscle and it is unaffected by cold acclimation.

Specificity

Uncoupling Protein 3 (UCP3), human. It is expected that the antibody will recognize both the long and short forms (30-33 kDa & 24-27 kDa) however splice variants are expected and precise reactivity profile has yet to be determined. Human UCP3 long form (UCP3L) is a 312 aa mitochondrial uncoupling protein (Boss et al. 1997; Vidal-Puig et al. 1997). It is only 57% and 73% homologous with human UCP1 and UCP2 respectively. Like other UCPs, UCP3 is predicted to contain 6 transmembrane domains. The UCP3S lacks the 6th transmembrane domain and is only 275 aa long (Vidal-Puig et al. 1997). UCP3 has preferential expression in muscle and it is unaffected by cold acclimation.

Immunogen

14 amino acid peptide sequence (designated UCP32) mapping near the C-terminus of human UCP3 (Boss et al. 1997). The UCP32 peptide has no significant homology with UCP1 or UCP2.

Application

Anti-Uncoupling Protein 3 Antibody is an antibody against Uncoupling Protein 3 for use in ELISA, IH & WB.
Research Category
Metabolism
Research Sub Category
Ion & Transport Channels
Western Blotting: 1-10 μg/mL using Chemiluminescence technique.

Immunohistochemistry: 2-20 μg/mL on formaldehyde fixed tissue.

ELISA: (1:10,000-1:100,000 using 50-100 ng UCP32 control peptide/well).

Optimal working dilutions must be determined by end user.

Target description

30-33 kDa & 24-27 kDa

Physical form

Affinity Purified immunoglobulin. Liquid in PBS with 0.1% BSA.
ImmunoAffinity Purified

Storage and Stability

Maintain for 1 year at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Analysis Note

Control
Muscle tissue, Blocking peptide for UCP3 is available (Catalogue No. AG769).

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

¿No encuentra el producto adecuado?  

Pruebe nuestro Herramienta de selección de productos.

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable


Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

¿Ya tiene este producto?

Encuentre la documentación para los productos que ha comprado recientemente en la Biblioteca de documentos.

Visite la Librería de documentos

Helena Gustafsson et al.
Journal of neuroscience research, 77(2), 285-291 (2004-06-24)
Uncoupling proteins (UCPs) have been reported to decrease the mitochondrial production of reactive oxygen species (ROS) by lowering the mitochondrial inner membrane potential (MMP). We have previously shown that UCP3 expression is positively regulated by insulin-like growth factor-1 (IGF-1). The
Xiaoyan Sun et al.
American journal of physiology. Endocrinology and metabolism, 285(3), E512-E520 (2003-05-02)
Uncoupling protein 3 (UCP3) is a member of the mitochondrial transporter superfamily that is expressed primarily in skeletal muscle. UCP3 is upregulated in various conditions characterized by skeletal muscle atrophy, including hyperthyroidism, fasting, denervation, diabetes, cancer, lipopolysaccharide (LPS), and treatment
K Sahlin et al.
American journal of physiology. Endocrinology and metabolism, 292(1), E223-E230 (2006-08-24)
The purpose of this study was to investigate fatty acid (FA) oxidation in isolated mitochondrial vesicles (mit) and its relation to training status, fiber type composition, and whole body FA oxidation. Trained (Vo(2 peak) 60.7 +/- 1.6, n = 8)
Fenofibrate activates the biochemical pathways and the de novo expression of genes related to lipid handling and uncoupling protein-3 functions in liver of normal rats.
Elena Silvestri, Pieter de Lange, Maria Moreno, Assunta Lombardi, Maurizio Ragni et al.
Biochimica et Biophysica Acta null
Anne D Hafstad et al.
Cardiovascular research, 83(3), 519-526 (2009-04-29)
Myocardial fatty acid (FA) oxidation is regulated acutely by the FA supply and chronically at the transcriptional level owing to FA activation of peroxisome proliferator-activated receptor-alpha (PPARalpha). However, in vivo administration of PPARalpha ligands has not been shown to increase

Nuestro equipo de científicos tiene experiencia en todas las áreas de investigación: Ciencias de la vida, Ciencia de los materiales, Síntesis química, Cromatografía, Analítica y muchas otras.

Póngase en contacto con el Servicio técnico