Saltar al contenido
Merck
Todas las fotos(1)

Documentos clave

AB1594P

Sigma-Aldrich

Anti-Serotonin Transporter Antibody

Chemicon®, from rabbit

Iniciar sesiónpara Ver la Fijación de precios por contrato y de la organización


About This Item

Código UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41

origen biológico

rabbit

Nivel de calidad

forma del anticuerpo

affinity purified immunoglobulin

tipo de anticuerpo

primary antibodies

clon

polyclonal

purificado por

affinity chromatography

reactividad de especies

rat, human

fabricante / nombre comercial

Chemicon®

técnicas

ELISA: suitable
western blot: suitable

Nº de acceso NCBI

Nº de acceso UniProt

Condiciones de envío

dry ice

modificación del objetivo postraduccional

unmodified

Información sobre el gen

Especificidad

Serotonin Transporter (SERT). An antibody raised against a similar peptide has been shown to react with a SERT in baculovirus cells expressing recombinant rat and human SERT. Antibody activity may be reduced considerably when detecting endogenous SERT from rat or human cells. SERT has been observed to migrate as 94 kDa (rat platelet), 80 kDa (pulmonary membranes) and 76 kDa (rat brain). This could be due to differences in postranslational membrane modifications (e.g. glycosylation).

Inmunógeno

A 15 amino acid sequence between transmembrane domain 7 and 8 of the rat serotonin transporter. This region is presumably from the extracellular domain.

Aplicación

Anti-Serotonin Transporter Antibody is an antibody against Serotonin Transporter for use in ELISA & WB.
Research Category
Neuroscience
Research Sub Category
Ion Channels & Transporters

Neuronal & Glial Markers
Western blot: 1-10 μg/mL


Cell pellets are resuspended in 100 μL lysis buffer (25 mM Tris, pH 7.6, 150 mM NaCl, 1 μg/mL aprotonin, 10 μg/mL leupeptin, 1 mM phenylmethylsulfonylfluoride, 1 mM sodium orthovanadate, 1 mM EDTA, and 1% Triton X-100). Samples were incubated for 1 hour at 4°C and were pelleted at high speed for 10 minutes at 4°C, and 100 μg protein per sample was subjected to 10% sodium dodecyl sulfate-polyacrylamide gel electrophoresis.





ELISA: 1:100,000 primary EIA using immunogen only (50-100 ng SERT peptide (Catalog Number AG297)/well). Not tested on purified protein at the stated dilutions.


Optimal working dilutions must be determined by end user.

Forma física

Affinity purified immunoglobulin. Liquid in 100 mM Tris, pH 7.5 with 0.2% BSA and 0.05% sodium azide.

Almacenamiento y estabilidad

Maintain at -20°C in undiluted aliquots for up to 12 months. Avoid repeated freeze/thaw cycles.

Otras notas

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Información legal

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

¿No encuentra el producto adecuado?  

Pruebe nuestro Herramienta de selección de productos.

Código de clase de almacenamiento

10 - Combustible liquids

Clase de riesgo para el agua (WGK)

WGK 2

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable


Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

¿Ya tiene este producto?

Encuentre la documentación para los productos que ha comprado recientemente en la Biblioteca de documentos.

Visite la Librería de documentos

Christophe Wersinger et al.
The European journal of neuroscience, 24(1), 55-64 (2006-08-03)
alpha-Synuclein (alpha-Syn), a protein primarily localized in the presynaptic compartment of neurons, is known to regulate dopaminergic neurotransmission by negatively modulating dopamine transporter activity and regulating its trafficking to or away from the cell surface. Given the considerable homology between
The cellular distribution of serotonin transporter is impeded on serotonin-altered vimentin network.
Ahmed, BA; Bukhari, IA; Jeffus, BC; Harney, JT; Thyparambil, S; Ziu, E; Fraer, M; Rusch et al.
Testing null
Christophe Wersinger et al.
Neuroscience letters, 453(3), 157-161 (2009-05-12)
Human alpha-synuclein (alpha-Syn) is instrumental in maintaining homeostasis of monoamine neurotransmitters in brain, through its trafficking, and regulation of the cell surface expression and, thereby, activity of dopamine, serotonin and norepinephrine transporters. Here we have investigated whether other members of
A M Thompson et al.
Developmental neuroscience, 27(1), 1-12 (2005-05-12)
To investigate the putative role of serotonin (5-HT) in auditory brainstem development, the expression of the 5-HT transporter (5-HTT) was evaluated in the normal mouse brainstem at 6 different postnatal ages. The brains of C3H/HeJ mice at birth (P0) and
Fate map of serotonin transporter-expressing cells in developing mouse thyroid.
Spina A, Rea S, De Pasquale V, Mastellone V, Avallone L, Pavone LM.
Anatomical Record (Hoboken, N.J. : 2007) (2007)

Nuestro equipo de científicos tiene experiencia en todas las áreas de investigación: Ciencias de la vida, Ciencia de los materiales, Síntesis química, Cromatografía, Analítica y muchas otras.

Póngase en contacto con el Servicio técnico