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Merck
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Documentos clave

537088

Millipore

Pronase® Protease, Streptomyces griseus, Nuclease-Free

Widely used broad-spectrum protease mixture

Sinónimos:

Nuclease-Free Protease, Streptomyces griseus, Pronase® Protease, Pronase® Protease, Streptomyces griseus

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About This Item

Número de CAS:
9036-06-0
Número MDL:
MFCD00132092
Código UNSPSC:
12352202
NACRES:
NA.54

descripción

RTECS - UK9595000

Formulario

lyophilized

actividad específica

≥70,000 proteolytic units/g dry wt

fabricante / nombre comercial

Calbiochem®

condiciones de almacenamiento

OK to freeze

solubilidad

water: 10 mg/mL

actividad extraña

Nuclease, none detected

Condiciones de envío

ambient

temp. de almacenamiento

2-8°C

Descripción general

PRONASE® Protease, Streptomyces griseus, nuclease-free is a non-specific protease that liquefies mucins and digests proteins to free amino acids. This mixture can be used to isolate living chondrocytes. It is most active at pH 7.0 but stable over wide ranges of pH and temperature. It has an optimal pH of 7.5. Pronase is less cytotoxic and produces more efficient dissociation than collagenase.

Aplicación

Pronase® Protease, Streptomyces griseus, nuclease-free has been used:
  • in single-cell preparations for cardiac side population (CSP) analysis
  • in aflatoxin plasma biomarker assay
  • to permeabilize Caenorhabditis elegans embryo for in vivo micrococcal nuclease digestion

Acciones bioquímicas o fisiológicas

Proteases catalyze the hydrolysis of proteins to form peptides and free amino acids. They also regulate the breakdown of fats and carbohydrates through many enzymatic cascades and activating proenzyme zymogens.

Advertencia

Toxicity: Harmful (C)

Definición de unidad

One unit is defined as the amount of enzyme that will liberate a digestion product equivalent to 25 µg of tyrosine per min at 40°C, pH 7.5.

Reconstitución

Unstable in solution; reconstitute just prior to use.

Otras notas

Note: 1 KU = 1000 units.

Información legal

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
PRONASE is a registered trademark of Merck KGaA, Darmstadt, Germany

Pictogramas

Health hazardExclamation mark
GHS08,GHS07

Palabra de señalización

Danger

Frases de peligro

H315,H319,H334,H335

Clasificaciones de peligro

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

Órganos de actuación

Respiratory system

Código de clase de almacenamiento

11 - Combustible Solids

Clase de riesgo para el agua (WGK)

WGK 2

Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Ehsan Pourkarimi et al.
eLife, 5 (2016-12-24)
The primary task of developing embryos is genome replication, yet how DNA replication is integrated with the profound cellular changes that occur through development is largely unknown. Using an approach to map DNA replication at high resolution in C. elegans
Mustafa Mahfuz et al.
Journal of exposure science & environmental epidemiology, 29(5), 655-662 (2018-09-07)
Dietary exposure to aflatoxin is implicated in growth faltering of children. Despite the high burden of childhood stunting in urban Bangladesh, there are no data on long-term exposure to aflatoxin. This study aimed to explore aflatoxin exposure levels in a
Industrial Biotechnology and Commodity Products
Comprehensive Biotechnology, 3, 587-603 null
Annette Meeson et al.
Stem cell reviews and reports, 9(5), 731-739 (2013-04-27)
Cardiac side population (CSP) cells, defined by their ability to efflux the vital dye Hoechst 33342, have been identified as putative cardiac stem cells based on their potential to give rise to both cardiomyocytes and endothelial cells. The CSP phenotype

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