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537060

Sigma-Aldrich

Propidium Iodide Solution

A convenient form of propidium iodide useful for flow cytometry studies.

Sinónimos:

Propidium Iodide Solution

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About This Item

Fórmula empírica (notación de Hill):
C27H34I2N4
Número de CAS:
25535-16-4
Peso molecular:
668.39
MDL number:
MFCD00011921
UNSPSC Code:
12352200
NACRES:
NA.47
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Quality Level

100

form

liquid

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze
protect from light

input

sample type intact cells

fluorescence

λex 536 nm
λem 617 nm

shipped in

ambient

storage temp.

2-8°C

SMILES string

[I-].I.[N+](CCCN1C2=CC(=N)C=CC2=C3C(=C1c4ccccc4)C=C(C=C3)N)(CC)(CC)C

InChI

1S/C27H33N4.2HI/c1-4-31(3,5-2)17-9-16-30-26-19-22(29)13-15-24(26)23-14-12-21(28)18-25(23)27(30)20-10-7-6-8-11-20;;/h6-8,10-15,18-19,29H,4-5,9,16-17,28H2,1-3H3;2*1H/q+1;;/p-1

InChI key

XJMOSONTPMZWPB-UHFFFAOYSA-M

General description

Membrane impermeant DNA intercalator. Useful for staining apoptotic cells and nuclei by flow cytometry. Useful in differentiating between apoptotic and necrotic cell death. Can be used together with common green fluorescent probes, e.g. fluorescein isothiocyanate. Excitation max.: ~536 nm; Emission max.: ~617 nm.

Biochem/physiol Actions

Cell permeable: no
Primary Target
Membrane impermeable DNA intercalator
Product does not compete with ATP.
Reversible: no

Warning

Toxicity: Standard Handling (A)

Physical form

50 µg/ml in PBS, pH 7.4.

Reconstitution

Following initial use, aliquot and freeze (-20°C).

Other Notes

Belloc, F., et al. 1994. Cytometry17, 59.
Cropmton, T., et al. 1992. Biochem. Biophys. Res. Commun.183, 532.
Darzynkiewicz, Z., et al. 1992. Cytometry13, 795.
de Caestecker, M.P., et al. 1992. J. Immunol. Methods154, 11.
Pollice, A.A., et al. 1992. Cytometry13, 432.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Protocolos

T-Cell Migration Assays Using Millicell® Cell Culture Inserts

Learn how to perform cell migration assays in vitro using Millicell® hanging cell culture inserts and the suspension T-cell lines Jurkat and primary CD4+ cells. Monitor migration by flow cytometry and EZ-MTT assays.

T-Cell Migration Assays Using Millicell® Cell Culture Inserts

Learn how to perform cell migration assays in vitro using Millicell® hanging cell culture inserts and the suspension T-cell lines Jurkat and primary CD4+ cells. Monitor migration by flow cytometry and EZ-MTT assays.

T-Cell Migration Assays Using Millicell® Cell Culture Inserts

Learn how to perform cell migration assays in vitro using Millicell® hanging cell culture inserts and the suspension T-cell lines Jurkat and primary CD4+ cells. Monitor migration by flow cytometry and EZ-MTT assays.

T-Cell Migration Assays Using Millicell® Cell Culture Inserts

Learn how to perform cell migration assays in vitro using Millicell® hanging cell culture inserts and the suspension T-cell lines Jurkat and primary CD4+ cells. Monitor migration by flow cytometry and EZ-MTT assays.

Questions

1–2 of 2 Questions  

  1. what is the concentration of the PI solution

    1 answer

    1. The concentration is 50 µg/ml in PBS, pH 7.4.

      Helpful?

  2. What is the recommended protocol for use/ working concentration?

    1 answer

    1. The working concentration must be determined by the end user.
      In the following reference: Cytometry. 1994 Sep 1;17(1):59-65. PMID: 7528124 DOI: 10.1002/cyto.990170108; 5ug/ML of PI was directly added in the culture medium for 30min. at 37C.

      Helpful?

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