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Key Documents

1.19613

Supelco

Superspher® 100 RP-18 (4 µm) HPLC Columns

HPLC sorbent

Sinónimos:

TM=["Superspher"] C18 sorbent for HPLC

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About This Item

UNSPSC Code:
41115709
NACRES:
SB.52

product name

Superspher® 100 RP-18,

material

stainless steel column

agency

suitable for USP L1

product line

Superspher®

feature

endcapped: no

parameter

60 °C max. temp.

technique(s)

HPLC: suitable
mass spectrometry (MS): suitable (Low - Medium)

matrix

fully porous particle

matrix active group

C18 bonding phase

particle size

4 μm

pore size

100 Å pore size

operating pH range

2-7.5

bulk density

200‑400 kg/m3

separation technique

reversed phase

storage temp.

no temp limit

General description

Superspher® RP-18 is a reliable and versatile traditionally produced spherical silica gel carriers with C-18 reversed phase properties. They are well suited for the separation of neutral, acidic and weak basic compounds. This high performance silica carrier with a particle size of 4µm yields an excellent pressure/separation performance ratio.

Application


  • Determination of retinol and retinyl esters in human plasma: This study utilized high-performance liquid chromatography with automated column switching and ultraviolet detection, highlighting the utility of the Superspher 100 RP-18 HPLC column in sensitive biochemical analyses (Hartmann et al., 2001).

  • Enantioselective assay for ketoprofen glucuronidation: This study underscores the column′s capability in pharmacokinetic research, using HPLC to analyze chiral anti-inflammatory drugs and their metabolites in liver microsomes (Chakir et al., 1994).

  • Rapid liquid chromatographic assay of glutathione in cultured cells: Employing the Superspher 100 RP-18 column, this research focused on rapid and effective glutathione quantification, demonstrating the column′s versatility in biomedicine (Leroy et al., 1993).

  • Assay of chloramphenicol acetyl transferase by high-performance liquid chromatography: This application further exemplifies the adaptability of Superspher® 100 RP-18 in biotechnology, offering precise measurement techniques for enzymatic activity studies (Burzio et al., 1988).

Analysis Note

Application test: passes test
Pressure: 50 - 70 bar
Theoretical Plates (N/m) (Anthracene): ≥ 105000
Symmetry (Anthracene): 0.8 - 1.4
Capacity factor (Anthracene): 4.0 - 5.5
separation factor (Acetophenone/Phenol, selectivity test): 1.90 - 2.15
separation factor (Acetophenone/Cinnamic acid, selectivity test): 1.20 - 1.37
separation factor (1-Naphtol/Phenol, selectivity test): 3.80 - 4.30
capacity factor (Toluene): 17.0 - 22.0

Legal Information

Superspher is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class

11 - Combustible Solids

wgk_germany

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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