05-678
Anti-ubiquityl-Histone H2A Antibody, clone E6C5
clone E6C5, Upstate®, from mouse
Sinónimos:
H2AUb, Histone H2A (ubiquityl), H2A histone family, member R, histone 1, H2aa, histone H2A, histone cluster 1, H2aa, H2AK119Ub1
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Productos recomendados
biological source
mouse
Quality Level
antibody form
purified antibody
antibody product type
primary antibodies
clone
E6C5, monoclonal
species reactivity
amphibian, mouse, human, frog, rat, monkey
manufacturer/tradename
Upstate®
technique(s)
ChIP: suitable
immunocytochemistry: suitable
western blot: suitable
isotype
IgM
NCBI accession no.
UniProt accession no.
shipped in
dry ice
target post-translational modification
unmodified
Gene Information
frog ... H2Ac1(594915)
human ... H2AC1(221613)
mouse ... H2Ac1(319163)
rat ... H2Ac1(24828)
Categorías relacionadas
General description
Histones are highly conserved proteins that serve as the structural scaffold for the organization of nuclear DNA into chromatin. Histones are modified post-translationally by acetylation, phosphorylation, methylation, and ubiquitination and these modifications regulate DNA transcription, repair, recombination, and replication. Ubiquitylation usually targets the substrate for degradation, although histones H2A and H2B are actually stabilized by a single ubiquitin conjugation. Histone ubiquitination has been correlated with DNA repair and transcription, cellular differentiation, cell cycle regulation, spermatogenesis, protein trafficking, and response to stress. Histone H2A is monoubiquitinated at Lys119 by the PRC-1L complex (Polycomb repressive complex 1-like), which includes Ring1, Ring2, Bmi1 and HPH2. Ubiquitinated H2A normally represents about 15% of H2A, but this value can be as high as 50% in active chromatin. Ubiquitinyl histone H2A has also been associated with transcriptional silencing of large chromatin regions and linked to Polycomb silencing so the function of ubiquitinated H2A remains undefined.
Specificity
Does not cross-react with army worm.
Other species not tested.
This antibody recognizes and is specific for monoubiquityl-Histone H2A (Lys119), Mr ~25 kDa.
Immunogen
AMA (human epithelial amnion) cell residual nuclear pellet portions.
Application
Immunocytochemistry:
This antibody has been reported by an independent laboratory to detect ubiquityl-Histone H2A in cells fixed with either 3% formaldehyde/0.1% Triton X-100 or methanol (Vassilev, A. 1995).
Chromatin Immunoprecipitation:
Reported by an independent laboratory (Wang, H. 2004).
This antibody has been reported by an independent laboratory to detect ubiquityl-Histone H2A in cells fixed with either 3% formaldehyde/0.1% Triton X-100 or methanol (Vassilev, A. 1995).
Chromatin Immunoprecipitation:
Reported by an independent laboratory (Wang, H. 2004).
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Research Sub Category
Histones
Histones
Use Anti-ubiquityl-Histone H2A Antibody, clone E6C5 (Mouse Monoclonal Antibody) has been published and validated for use in ChIP, ICC, WB to detect ubiquityl-Histone H2A also known as H2AUb, Histone H2A (ubiquityl), histone H2A.
Quality
Routinely evaluated by western blot on H2A in acid extracts from HeLa and 10T1/2 cells.
Western Blot Analysis:
A 1:500-1:2000 dilution of this lot detected ubiquityl-Histone H2A in acid extracts from HeLa and 10T1/2 cells.
Western Blot Analysis:
A 1:500-1:2000 dilution of this lot detected ubiquityl-Histone H2A in acid extracts from HeLa and 10T1/2 cells.
Target description
25 kDa
Physical form
Format: Purified
Purified mouse monoclonal IgM in buffer containing PBS with 0.05% sodium azide before the addition of glycerol to 30%. Liquid at -20ºC.
Storage and Stability
Stable for 1 year at -20°C from date of receipt. For maximum recovery of product, centrifuge the vial prior to removing the cap.
Analysis Note
Control
Acid extracts of HeLa cells.
Acid extracts of HeLa cells.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class
12 - Non Combustible Liquids
wgk_germany
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificados de análisis (COA)
Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»
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Genome-wide uH2A localization analysis highlights Bmi1-dependent deposition of the mark at repressed genes.
Kallin, EM; Cao, R; Jothi, R; Xia, K; Cui, K; Zhao, K; Zhang, Y
PLoS Genetics null
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Archives of biochemistry and biophysics, 518(2), 103-110 (2012-01-12)
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The Journal of biological chemistry, 289(50), 34862-34870 (2014-10-23)
Ubiquitin-specific peptidase 42 (USP42) is a deubiquitylating enzyme that can target p53 and contribute to the stabilization of p53 in response to stress. We now show that USP42 can also regulate transcription independently of p53. USP42 co-localized with RNA polymerase
MOF and H4 K16 acetylation play important roles in DNA damage repair by modulating recruitment of DNA damage repair protein Mdc1.
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