05-321MG

Anti-Phosphotyrosine Antibody, clone 4G10^®

clone 4G10®, Upstate^®, from mouse

• Sinónimos: Clone 4G10 Anti-Phosphotyrosine, Phosphotyrosine Detection Antibody
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Propiedades

• biological source: mouse
• Quality Level: 100
• antibody form: purified from hybridoma cell culture
• antibody product type: primary antibodies
• clone: 4G10®, monoclonal
• species reactivity (predicted by homology): all
• manufacturer/tradename: Upstate^®
• technique(s): immunoprecipitation (IP): suitable; western blot: suitable
• isotype: IgG2bκ
• shipped in: wet ice
• target post-translational modification: phosphorylation (pTyr)

Descripción

Application

Anti-Phosphotyrosine Antibody, clone 4G10 detects level of Phosphotyrosine & has been published & validated for use in IP & WB.

Quality

Routinely evaluated on EGF-treated human A431 carcinoma cells.

Western Blot Analysis:
0.5-2 μg/mL of this lot detected tyrosine-phosphorylated proteins in a modified RIPA lysate from EGF-treated human A431 carcinoma cells (Cohen, B., 1990; Druker, B. J., 1989; Kanakura, Y., 1991).

Target description

Dependent upon the molecular weight of the tyrosine phosphorylated protein being detected.

Physical form

Format: Purified

Purified mouse monoclonal IgG2bκ in buffer containing PBS, pH 7.4. Liquid at 2-8ºC.
IgG2bκ mouse monoclonal antibody produced in vitro by mouse-mouse hybridoma 4G10 (FOX-NY [NS-1 derivative] myeloma x spleen cells).

Analysis Note

Control
Positive Antigen Control: Catalog #12-302, EGF-stimulated A431 cell lysate. Add 2.5µL of 2-mercaptoethanol/100µL of lysate and boil for 5 minutes to reduce the preparation. Load 20µg of reduced lysate per lane for minigels.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

4G10 is a registered trademark of Upstate Group, Inc.

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Información de seguridad

• Storage Class: 12 - Non Combustible Liquids
• wgk_germany: WGK 2
• flash_point_f: Not applicable
• flash_point_c: Not applicable