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X4126

Sigma-Aldrich

Xylene Cyanol FF

BioReagent, for molecular biology

Synonym(s):

Acid blue 147, Cyanol FF, XC

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About This Item

Empirical Formula (Hill Notation):
C25H27N2NaO6S2
CAS Number:
2650-17-1
Molecular Weight:
538.61
Colour Index Number:
42135
EC Number:
220-167-5
MDL number:
MFCD00019481
UNSPSC Code:
12171500
PubChem Substance ID:
57654732
NACRES:
NA.52

grade

for molecular biology

product line

BioReagent

form

powder

technique(s)

nucleic acid detection: suitable

solubility

H2O: complete 1 mg/mL, clear, blue

suitability

suitable for nucleic acid staining

storage temp.

room temp

SMILES string

[Na+].CCNc1ccc(cc1C)C(=C2/C=CC(=N\CC)\C(C)=C2)\c3ccc(cc3S(O)(=O)=O)S([O-])(=O)=O

InChI

1S/C25H28N2O6S2.Na/c1-5-26-22-11-7-18(13-16(22)3)25(19-8-12-23(27-6-2)17(4)14-19)21-10-9-20(34(28,29)30)15-24(21)35(31,32)33;/h7-15,26H,5-6H2,1-4H3,(H,28,29,30)(H,31,32,33);/q;+1/p-1/b25-19-,27-23-;

InChI key

VVLFAAMTGMGYBS-KRQUPCAFSA-M

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General description

Xylene cyanol is often used as a tracking dye during agarose or polyacrylamide gel electrophoresis. It has a slight negative charge and will migrate the same direction as DNA, allowing the user to monitor the progress of molecules moving through the gel. The rate of migration varies with gel composition.

Application

Xylene Cyanol FF is used as a:
  • histological stain and as tracker dye in DNA sequencing and electrophoresis
  • gel-loading dye
  • formamide RNA loading dye
  • tracking dye in native polyacrylamide gel electrophoresis (PAGE) loading buffer

Biochem/physiol Actions

Xylene Cyanol FF acts as nucleic acid stain.

Reconstitution

A 6x Gel Loading Buffer can be made using standard recipes, including 0.05% xylene cyanol.

Pictograms

Exclamation mark
GHS07

Signal Word

Warning

Hazard Statements

H319

Hazard Classifications

Eye Irrit. 2

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Single nucleotide polymorphism detection by polymerase chain reaction-restriction fragment length polymorphism
<BIG>Ota M, et al.</BIG>
Nature Protocols, 2857-2857 (2007)
P N Hengen
Trends in biochemical sciences, 20(5), 202-203 (1995-05-01)
Methods and reagents is a unique monthly column that highlights current discussions in the newsgroup bionet.molbio.methods-reagents, available on the Internet. This month's column describes how to make an electrophoresis system from common household items. For details on how to partake
Cell-internalization SELEX: method for identifying cell-internalizing RNA aptamers for delivering siRNAs to target cells
<BIG>Thiel WH, et al.</BIG>
Methods in Molecular Biology, 187-199 (2015)
Design and self-assembly of siRNA-functionalized RNA nanoparticles for use in automated nanomedicine
<BIG>Afonin KA, et al.</BIG>
Nature Protocols, 6, 2022-2022 (2011)
Patrick S Aranda et al.
Electrophoresis, 33(2), 366-369 (2012-01-10)
RNA-based applications requiring high-quality, non-degraded RNA are a foundational element of many research studies. As such, it is paramount that the integrity of experimental RNA is validated prior to cDNA synthesis or other downstream applications. In the absence of expensive
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