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SEQR

Sigma-Aldrich

SeqPlex RNA Amplification Kit

For use with high throughput sequencing technologies

Synonym(s):

WGA kit, Whole transcriptome amplification

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About This Item

UNSPSC Code:
41121800
NACRES:
NA.55

Quality Level

200

technique(s)

whole genome amplification: suitable

shipped in

wet ice

storage temp.

−20°C

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General description

The SeqPlex™ RNA Amplification Kit for whole transcriptome amplification (WTA) is designed to facilitate next-generation sequencing (NGS) from small quantities or from degraded/highly fragmented RNA (e.g. RNA from formalin-fixed paraffin-embedded (FFPE) tissue samples). The SeqPlex kit allows the user to pre-amplify these and other small quantity/highly fragmented RNA samples for input into a NGS workflow. It also facilitates the amplification of non-polyA tailed RNA isolated from tissue, cultured cells, formalin-fixed samples, or serum while maintaining patterns of differential expression found in the unamplified sample.

Application

SeqPlex™ RNA Amplification Kit has been used to amplify poly(A)-selected RNA.

Features and Benefits

  • Low quantities of total RNA random priming technology amplifies fragmented or intact RNA from all sources including FFPE and RIP.
  • Semi-degenerate library primer design for more complete transcriptome coverage and efficient priming.
  • No need to fragment DNA before sequencing.
  • Amplifies ds-cDNA in 8 hours or less.
  • Compatible with all next generation sequencing platforms except Pacific Bioscience.

Other Notes

SEQR-500RXN is manufactured on-demand. Contact us for more information.

Legal Information

SeqPlex is a trademark of Sigma-Aldrich Co. LLC

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Description
Pricing

Storage Class Code

11 - Combustible Solids

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Grégory Seumois et al.
Nature immunology, 15(8), 777-788 (2014-07-07)
A characteristic feature of asthma is the aberrant accumulation, differentiation or function of memory CD4(+) T cells that produce type 2 cytokines (TH2 cells). By mapping genome-wide histone modification profiles for subsets of T cells isolated from peripheral blood of
Kathryn D Tuttle et al.
Nature communications, 9(1), 2650-2650 (2018-07-10)
During development in the thymus, invariant natural killer T (iNKT) cells commit to one of three major functionally different subsets, iNKT1, iNKT2, and iNKT17. Here, we show that T cell antigen receptor (TCR) signal strength governs the development of iNKT
Giuliana P Mognol et al.
Proceedings of the National Academy of Sciences of the United States of America, 114(13), E2776-E2785 (2017-03-12)
T-cell exhaustion is a progressive loss of effector function and memory potential due to persistent antigen exposure, which occurs in chronic viral infections and cancer. Here we investigate the relation between gene expression and chromatin accessibility in CD8+ tumor-infiltrating lymphocytes
Isaac Engel et al.
Nature immunology, 17(6), 728-739 (2016-04-19)
Natural killer T cells (NKT cells) have stimulatory or inhibitory effects on the immune response that can be attributed in part to the existence of functional subsets of NKT cells. These subsets have been characterized only on the basis of
DNA Sequencing Research Group (DSRG): Evaluation of RNA Amplification Kits at Subnanogram Input Amounts of Total RNA for RNA-Seq
Nicolet C, et al.
Journal of biomolecular techniques : JBT, 24, S70-S70 (2013)
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Protocols

SEQR - SeqPlex RNA Amplification Kit Protocol

The SeqPlex RNA Amplification kit provides a method for amplification of total RNA or isolated mRNA prior to entry into the workflows of the commonly used deep sequencing platforms.

SEQR - SeqPlex RNA Amplification Kit Protocol

The SeqPlex RNA Amplification kit provides a method for amplification of total RNA or isolated mRNA prior to entry into the workflows of the commonly used deep sequencing platforms.

SEQR - SeqPlex RNA Amplification Kit Protocol

The SeqPlex RNA Amplification kit provides a method for amplification of total RNA or isolated mRNA prior to entry into the workflows of the commonly used deep sequencing platforms.

SEQR - SeqPlex RNA Amplification Kit Protocol

The SeqPlex RNA Amplification kit provides a method for amplification of total RNA or isolated mRNA prior to entry into the workflows of the commonly used deep sequencing platforms.

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