SAB4200321

Anti-PTPRF (C-terminal) antibody produced in rabbit

IgG fraction of antiserum

• Synonym(s): Anti-LAR, Anti-Leukocyte antigen-related tyrosine phosphatase, Anti-Leukocyte common antigen-related tyrosine phosphatase, Anti-Tyrosine protein phosphatase receptor type F
• UNSPSC Code: 12352203
• NACRES: NA.44

Properties

• biological source: rabbit
• Quality Level: 200
• conjugate: unconjugated
• antibody form: IgG fraction of antiserum
• antibody product type: primary antibodies
• clone: polyclonal
• form: buffered aqueous solution
• mol wt: antigen ~150 kDa; antigen ~210 kDa; antigen ~85 kDa
• species reactivity: human
• technique(s): immunoprecipitation (IP): 5-10 μL using lysates of HEK-293T cells over-expressing human PTPRF.; western blot: 1:500-1:1000 using lysates of HEK-293T cells over-expressing human PTPRF.
• UniProt accession no.: P10586
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... PTPRF(5792) ; mouse ... Ptprf(19268) ; rat ... Ptprf(360406)

Description

General description

PTPRF or leukocyte common antigen-related protein (LAR) is a widely expressed protein tyrosine phosphatase with an extracellular receptor region that resembles a cell adhesion molecule.

Protein tyrosine phosphatase, receptor type F (PTPRF) consists of noncovalently bound subunits designated as E (extracellular) and P (phosphatase) subunits that are derived from a precursor protein by proteolytic processing. The E subunit (150 kDa) has three immunoglobulin-like domains and eight fibronectin III-like domains, and the P subunit (85 kDa) has a short extracellular domain, a transmembrane segment and tandem cytoplasmic PTP catalytic domains.

Specificity

The antibody recognizes both the extracellular and the cytoplasmic subunits of human PTPRF. Anti-PTPRF (C-terminal) antibody is specific for the P- subunit and the whole PTPRF protein from Human origin. Detection of the PTPRF bands by immunoblotting is specifically inhibited by the immunizing peptide.

Application

Anti-PTPRF (C-terminal) antibody produced in rabbit has been used in an enzyme based assay of protein tyrosine phosphatase 1 (PTP1).

The antibody may be used in several immunochemical techniques including immunoblotting (∼210, ∼150, and ∼85 kDa) and immunoprecipitation.

Biochem/physiol Actions

Protein tyrosine phosphatase, receptor type F (PTPRF) removes phosphate group from β-catenin, an event that may subsequently facilitate cell-cell adhesion and ensure the stability of the cadherin complex. This phosphatase has also been implicated in various cellular processes such as neurite growth, nerve regeneration, actin remodeling and regulation of insulin function. Insulin receptor, epidermal growth factor receptor (EGF) receptor, hepatocyte growth factor (HGF) receptor and rearranged during transfection (RET) have been reported as substrates of PTPRF.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable