S7776
Starch Azure
Insoluble corn starch covalently linked with Remazol Brilliant Blue R
Synonym(s):
Starch–Remazol brilliant blue R, RBB-Starch, Remazol brilliant blue R dyed starch, Starch Azure
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About This Item
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Quality Level
form
powder
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General description
Starch is an insoluble starch substrate specific for α-amylase and not for other exo enzymes. α-amylase catalysis of starch azure solubilization is measured at 595 nm.
Application
Starch Azure has been used as a substrate
- in the screening of α-amylase activity in decontaminated malted flour at varying pH and temperature
- for monitoring the inhibitory activity of Physalis peruviana extracts
- for thermostable α-amylase extracted from deep sea and acidic soil
Substrates
Substrate for colorimetric determination of α-amylase.
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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The Journal of Biological Chemistry, 277(29), 26501-26507 (2002)
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Mestres C, et al.
Food Research International, 100, 102-111 (2017)
Inhibitory effects of an extract of fruits of Physalis peruviana on some intestinal carbohydrases
Rey D, et al.
Revista Colombiana de Ciencias Quimico-Farmaceuticas, 44(1), 72-89 (2015)
The use of starch azure for measurement of alpha-amylase activity
Lehoczki G, et al.
Carbohydrate Polymers, 183, 263-266 (2018)
Gábor Lehoczki et al.
Carbohydrate polymers, 183, 263-266 (2018-01-22)
Despite being widely used, there is no standard protocol for α-amylase activity measurement with starch azure substrate. Boiling pre-treatment of starch azure suspension increased the reaction rate of hydrolysis catalysed by human salivary α-amylase (HSA) or porcine pancreatic α-amylase (PPA)
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