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S7651

Sigma-Aldrich

Sphingomyelinase from Bacillus cereus

lyophilized powder, ≥100 units/mg protein

Synonym(s):

Sphingomyelin choline phosphohydrolase, Sphingomyelin phosphodiesterase

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About This Item

CAS Number:
Enzyme Commission number:
EC Number:
MDL number:
UNSPSC Code:
12352204
NACRES:
NA.54

Pricing and availability is not currently available.

form

lyophilized powder

Quality Level

specific activity

≥100 units/mg protein

mol wt

36873 Da

composition

Protein, ~10% Lowry

storage temp.

2-8°C

Gene Information

Bacillus cereus ATCC 14579 ... BC0671(1203022)

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form

solid

form

solid

form

-

form

film, solid

Quality Level

100

Quality Level

200

Quality Level

-

Quality Level

100

manufacturer/tradename

Calbiochem®

manufacturer/tradename

-

manufacturer/tradename

PhytoLab

manufacturer/tradename

Calbiochem®

storage temp.

−20°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

−20°C

solubility

DMSO: 25 mg/mL, warm ethanol: 25 mg/mL

solubility

DMF: soluble, DMSO: soluble, ethanol: soluble

solubility

-

solubility

DMSO: 25 mg/mL, ethanol: 25 mg/mL

General description

Sphingomyelinase from Bacillus cereus (Bc-Smase) is a Mg2+-containing metalloenzyme.[1] Bacillus cereus sphingomyelinase induces hemolysis of sheep erythrocytes.[2]
Sphingomyelinase modulates adenosine triphosphate (ATP)-independent endocytosis of fibroblast and large vesicles of macrophages.[3] Sphingomyelinase causes hemolysis of human erythrocytes. It is also a potent virulence factor leading to invasive diseases.[4]

Application

Sphingomyelinase from Bacillus cereus has been used:
  • in the measurement of membrane capacitance in brain slices[3]
  • for testing and confirming its silkworm-killing activity[4]
  • in enzyme activity assay and for elucidating its effect on hydrolysis of sphingomyelin in complex lipid mixtures[5]

Sphingomyelinase has been used in a study to investigate skeletal manifestations in pediatric and adult patients with Niemann Pick disease. [6]

Biochem/physiol Actions

Initiates the formation of sphingomyelin-based second messengers. Activates MAPK (mitogen-activated protein kinase) and SAPKs (stress-activated protein kinases); generates ceramide from sphingomyelin.

Unit Definition

One unit will hydrolyze 1.0 μmole of TNPAL-sphingomyelin per min at pH 7.4 at 37 °C.

Physical form

Lyophilized powder containing potassium phosphate buffer salts and stabilizer.

Pictograms

Exclamation mark

Signal Word

Warning

Hazard Statements

Hazard Classifications

Skin Sens. 1

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

  • Certificates of Analysis (COA)

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    Masataka Oda
    Yakugaku zasshi : Journal of the Pharmaceutical Society of Japan, 129(10), 1233-1238 (2009-10-03)
    Bacillus cereus is one that causes of opportunistic human infections. Sphingomyelinase produced by B. cereus is assumed a virulence factor for the infection. Sphingomyelinase from Bacillus cereus (Bc-SMase) is Mg(2+)-containing metalloenzyme. Bc-SMase is a family of neutral SMase (nSMase) and
    Masataka Oda et al.
    Biochimica et biophysica acta, 1798(6), 1073-1080 (2010-03-11)
    Bacillus cereus sphingomyelinase (Bc-SMase) induces hemolysis of sheep erythrocytes which contain large amounts of sphingomyelin. We investigated the mechanism of this hemolysis in comparison to that induced by Clostridium perfringens alpha-toxin. Pertussis toxin, a Gi-specific inhibitor, N-oleoylethernolamine, a ceramidase inhibitor
    Lipid bilayers containing sphingomyelins and ceramides of varying N-acyl lengths: a glimpse into sphingolipid complexity
    Jimenez-Rojo N, et al.
    Biochimica et Biophysica Acta - Biomembranes, 1838(1), 456-464 (2014)
    Purification of a soil bacteria exotoxin using silkworm toxicity to measure specific activity
    Usui K, et al.
    Microbial Pathogenesis, 46(2), 59-62 (2009)
    Melissa Wasserstein et al.
    Journal of inherited metabolic disease, 36(1), 123-127 (2012-06-22)
    Niemann-Pick disease (NPD) due to acid sphingomyelinase deficiency is a lipid storage disease resulting from the accumulation of sphingomyelin, predominantly within cells of the monocyte-macrophage system. In contrast to other lysosomal storage disorders, skeletal involvement in NPD has not been

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