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S2890

Sigma-Aldrich

Streptavidin−Alkaline Phosphatase from Streptomyces avidinii

lyophilized powder, ≥700 DEA units/mg protein

Synonym(s):

Streptavidin-AP, Streptavidin−AP from Streptomyces avidinii

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About This Item

MDL number:
MFCD00132384
UNSPSC Code:
12352203
NACRES:
NA.46

conjugate

alkaline phosphatase conjugate

Quality Level

200

form

lyophilized powder

specific activity

≥700 DEA units/mg protein

composition

Protein, ≥40% biuret

storage temp.

−20°C

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General description

Streptavidin, a 58 kDa protein, is a homotetramer purified from Streptomyces avidinii. It has a high affinity for biotin. Streptavidin has been conjugated with either peroxidase or alkaline phosphatase (ALP). These enzymes are used as protein conjugates because of their comparative stability and a wide range of suitable substrates for different applications. ALP conjugates to streptavidin in immunoassays.

Application

Streptavidin-alkaline phosphatase from Streptomyces avidinii has been used in enzyme-linked immune absorbent spot (ELISpot) to detect interferon (IFN-γ) producing cells.
The optimal working dilution should be determined empirically using a range of dilutions from a 1 mg/ml stock in buffer.

Unit Definition

One alkaline phosphatase unit will hydrolyze 1.0 μmole of p-nitrophenyl phosphate per min at pH 9.8 at 37 °C.

Physical form

Lyophilized powder containing trehalose with EPPS, phosphate and traces of magnesium chloride and zinc chloride.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Bystander activation of irrelevant CD4+ T cells following antigen-specific vaccination occurs in the presence and absence of adjuvant
van Aalst S, et al.
PLoS ONE, 12(5), e0177365-e0177365 (2017)
Microbial Resource Conservation: Conventional to Modern Approaches (2018)
Ola Blixt et al.
The Journal of biological chemistry, 278(33), 31007-31019 (2003-05-30)
Ten of the 11 known human siglecs or their murine orthologs have been evaluated for their specificity for over 25 synthetic sialosides representing most of the major sequences terminating carbohydrate groups of glycoproteins and glycolipids. Analysis has been performed using
Development and validation of sandwich quantitative ELISA prototypebased on the bovine IFNg for the detection of cellular immunity
Genc O, et al.
Turkish Journal of Veterinary and Animal Sciences, 39(6), 724-729 (2015)
An allosteric-probe for detection of alkaline phosphatase activity and its application in immunoassay
Guo J, et al.
Frontiers in Chemistry, 6, 618-618 (2018)
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Protocols

Diethanolamine Assay for the Enzymatic Assay of Alkaline Phosphatase (EC 3.1.3.1)

Enzymatic Assay of Alkaline Phosphatase, Diethanolamine Assay (EC 3. 1. 3. 1)

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