R1753
Ribonucleic acid, transfer from Escherichia coli
Type XX, Strain W, lyophilized powder
Synonym(s):
Transfer RNA, tRNA
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Application
Ribonucleic acid, transfer from Escherichia coli, Type XX, Strain W may be used as a starting material for the purification of specific amino acyl-tRNA species by methods such as liquid column chromatography or high-performance liquid chromatography (HPLC).
Unit Definition
One unit will yield an A260 of 1.0 in 1.0 mL of water (1 cm light path).
Analysis Note
Each lot is evaluated for its amino acid acceptor activity using aminoacyl-tRNA synthetase and Arg.
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Roya Pedram Fatemi et al.
Journal of biomolecular screening, 20(9), 1132-1141 (2015-07-16)
Long non-protein coding RNAs (lncRNAs) are an important class of molecules that help orchestrate key cellular events. Although their functional roles in cells are not well understood, thousands of lncRNAs and a number of possible mechanisms by which they act
Miguel López-Estepa et al.
PloS one, 10(4), e0118606-e0118606 (2015-04-22)
Cyclic N6-threonylcarbamoyladenosine ('cyclic t6A', ct(6)A) is a non-thiolated hypermodification found in transfer RNAs (tRNAs) in bacteria, protists, fungi and plants. In bacteria and yeast cells ct(6)A has been shown to enhance translation fidelity and efficiency of ANN codons by improving
Kirill Kondratov et al.
Journal of extracellular vesicles, 9(1), 1743139-1743139 (2020-04-29)
The aim of this study was to investigate cell source-dependent nucleic acids repertoire of diverse subpopulations of plasma extracellular vesicles (EVs). Blood plasma from nine healthy volunteers was used for the analysis. Samples of EVs were obtained by differential centrifugation
Fuxiang Wei et al.
Nature communications, 11(1), 4902-4902 (2020-10-01)
Living cells and tissues experience various complex modes of forces that are important in physiology and disease. However, how different force modes impact gene expression is elusive. Here we apply local forces of different modes via a magnetic bead bound
Thomas E Sladewski et al.
eLife, 7 (2018-06-27)
We investigated the role of full-length Drosophila Bicaudal D (BicD) binding partners in dynein-dynactin activation for mRNA transport on microtubules. Full-length BicD robustly activated dynein-dynactin motility only when both the mRNA binding protein Egalitarian (Egl) and K10 mRNA cargo were
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