P7208

Pertussis toxin

from Bordetella pertussis, lyophilized powder, protein endotoxin

• Synonym(s): Histamine-sensitizing factor, IAP, Islet Activating Protein, PTX, Pertussigen
• CAS Number: 70323-44-3
• MDL number: MFCD00131841
• UNSPSC Code: 12352200
• NACRES: NA.77

Properties

• product name: Pertussis toxin from Bordetella pertussis, lyophilized powder
• form: lyophilized powder
• Quality Level: 200
• storage temp.: 2-8°C
• Gene Information: Bordetella pertussis Tohama I ... ptxA(2665068) , ptxB(2665069) , ptxC(2665408)

Description

General description

Pertussis toxin (PT) is a multi-subunit complex toxin that possesses one active subunit(A) and five binding subunits (B5). PT enters the mammalian cells via endocytosis by attaching itself to the glycosylated molecules on the surface of these cells.

Application

Pertussis toxin from Bordetella pertussis has been used: as a blocker of the G_αi subunit of the chemokine (CXCR4) receptor to measure the intracellular Ca²⁺ in breast cancer cell lines, to characterize the Gi signaling involved in ADP-induced tissue factor (TF) and P-selectin exposure, to induce autoimmune vasculitis in a rat model

The toxin is released from B. pertussis in an inactive form. When the pertussis toxin B oligomer binds to the cell membrane, the S1 subunit of its A protomer becomes activated, perhaps through the action of glutathione and ATP. A protocol for activating pertussis toxin in vitro is given by Kaslow, et al.

Biochem/physiol Actions

Pertussis toxin catalyzes the ADP-ribosylation of the α subunits of the heterotrimeric guanine nucleotide regulatory proteins G_i, G_o, and G_t. This prevents the G protein heterotrimers from interacting with receptors, thus blocking their coupling and activation. Since the G_α subunits remain in their GDP-bound, inactive state, they are unable to inactivate adenylyl cyclase or open K⁺ channels.

Features and Benefits

This compound is a featured product for Cyclic Nucleotide research. Click here to discover more featured Cyclic Nucleotide products. Learn more about bioactive small molecules for other areas of research at sigma.com/discover-bsm.

This compound is featured on the G Proteins (Heterotrimeric) page of the Handbook of Receptor Classification and Signal Transduction. To browse other handbook pages, click here.

Physical form

Lyophilized powder containing sodium chloride and sodium phosphate buffer salts

Safety Information

• Pictograms: GHS07
• Signal Word: Warning
• Hazard Statements: H315,H319,H335
• Precautionary Statements: P261 - P264 - P271 - P280 - P302 + P352 - P305 + P351 + P338
• Hazard Classifications: Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3
• Target Organs: Respiratory system
• Storage Class Code: 13 - Non Combustible Solids
• WGK: WGK 2
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves