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P7130

Sigma-Aldrich

Puromycin aminonucleoside

Synonym(s):

3′-Amino-3′-deoxy-N6,N6-dimethyladenosine

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About This Item

Empirical Formula (Hill Notation):
C12H18N6O3
CAS Number:
58-60-6
Molecular Weight:
294.31
Beilstein:
93902
EC Number:
200-388-3
MDL number:
MFCD00063462
UNSPSC Code:
51281912
PubChem Substance ID:
24898823
NACRES:
NA.85

form

powder

Quality Level

300

solubility

H2O: soluble 50 mg/mL

antibiotic activity spectrum

Gram-positive bacteria
neoplastics
parasites

Mode of action

protein synthesis | interferes

storage temp.

2-8°C

SMILES string

CN(C)c1ncnc2n(cnc12)[C@@H]3O[C@H](CO)[C@H](N)[C@@H]3O

InChI

1S/C12H18N6O3/c1-17(2)10-8-11(15-4-14-10)18(5-16-8)12-9(20)7(13)6(3-19)21-12/h4-7,9,12,19-20H,3,13H2,1-2H3/t6-,7+,9+,12-/m1/s1

InChI key

RYSMHWILUNYBFW-VENHTOENSA-N

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General description

Puromycin aminonucleoside is the aminonucleoside portion of the antibiotic puromycin. It is useful in nephrology research, like studies of focal and segmental glomerulosclerosis and in the induction of nephrosis in rats. The excretion of sodium and nitrite (NOx) metabolites in rats with puromycin aminonucleoside-induced nephrotic syndrome are studied. Puromycin aminonucleoside-induced nephrosis in rats has been studied with respect to the production of reactive oxygen species during the acute phase. Puromycin aminonucleoside is used to probe endothelial glycosaminoglycan synthesis in cultured glomerular endothelial cells and their relation to cell permeability.

Application

Puromycin aminonucleoside has been used:
  • as a selection marker for the infected cells for the transfection of lentivirus
  • as a component of fresh medium for transfection and infection assays
  • to subcutaneously induce puromycin aminonucleoside nephrosis to investigate the mRNA and protein levels of nephrin and podocin before the onset of proteinuria

Biochem/physiol Actions

Puromycin aminonucleoside is used to study human glomerular disease by inducing damage of murine glomerular podocytes and is used to study glomerular function and morphology.

Other Notes

Keep container tightly closed in a dry and well-ventilated place.

Pictograms

Health hazard
GHS08

Signal Word

Warning

Hazard Statements

H373

Hazard Classifications

STOT RE 2

Target Organs

Kidney

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Ido Refaeli et al.
Scientific reports, 10(1), 9419-9419 (2020-06-12)
Dominant and recessive mutations in podocalyxin (PODXL) are associated with human kidney disease. Interestingly, some PODXL mutations manifest as anuria while others are associated with proteinuric kidney disease. PODXL heterozygosity is associated with adult-onset kidney disease and podocalyxin shedding into
Ramzi Khalil et al.
The Journal of pathology, 247(2), 177-185 (2018-10-24)
Dynamin plays an essential role in maintaining the structure and function of the glomerular filtration barrier. Specifically, dynamin regulates the actin cytoskeleton and the turnover of nephrin in podocytes, and knocking down dynamin expression causes proteinuria. Moreover, promoting dynamin oligomerization
Yuqiu Lu et al.
Kidney international, 92(5), 1119-1129 (2017-07-16)
Gene expression differs substantially among individual cells of the same type. We speculate that genes that are expressed in all but a portion of cells of a given cell type would be likely essential and required for either the cell
G N Marinides et al.
Kidney international, 37(2), 749-757 (1990-02-01)
The effects of dietary protein and converting enzyme inhibition (CEI) on chronic puromycin aminonucleoside nephropathy (PAN) were studied. PAN was induced with seven SQ injections of puromycin aminonucleoside 20 mg/kg over 10 weeks in male Sprague-Dawley rats. The rats were
Anastasia Korolj et al.
Lab on a chip, 18(20), 3112-3128 (2018-09-29)
Most kidney diseases begin with abnormalities in glomerular podocytes, motivating the need for podocyte models to study pathophysiological mechanisms and new treatment options. However, podocytes cultured in vitro face a limited ability to maintain appreciable extents of differentiation hallmarks, raising
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