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MAK015

Sigma-Aldrich

Glucose-6-Phosphate Dehydrogenase Activity Assay Kit

sufficient for 100 colorimetric tests

Synonym(s):

G-6-PD Activity Assay Kit

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About This Item

UNSPSC Code:
12161503
NACRES:
NA.84

usage

sufficient for 100 colorimetric tests

detection method

colorimetric

relevant disease(s)

cancer; hematological disorder

storage temp.

−20°C

Gene Information

human ... H6PD(9563)
mouse ... H6PD(100198)
rat ... H6PD(298655)

General description

Glucose-6-phosphate dehydrogenase (G6PDH) catalyzes the conversion of glucose-6-phosphate to 6-phosphoglucono-δ-lactone, the first and rate-limiting step of the pentose phosphate pathway (PPP). The PPP pathway critical for maintaining the co-enzyme nicotinamide adenine dinucleotide phosphate (NADPH) and for the production of pentose sugars. The NADPH produced is critical for redox regulation via the regeneration of GSH and for providing reducing equivalents for fatty acid biosynthesis. Deficiencies in G6PDH predisposes individuals to non-immune hemolytic anemia.

Application

Glucose-6-phosphate dehydrogenase assay kit has been used in the determination of glucose-6-phosphate dehydrogenase (G6PDH) activity and to measure PDH (pyruvate dehydrogenase).

Suitability

Suitable for the measurement of Glucose-6-phosphate dehydrogenase (G6PDH) activity in various tissues and cells.

Principle

In the glucose-6-phosphate dehydrogenase assay kit, glucose-6-phosphate is oxidized to generate a product which is specifically detected by colorimetric (450 nm) assay. The G6PDH Assay Kit can detect G6PDH activity as low as 0.04mU G6PDH per well.

related product

Product No.
Description
Pricing

Pictograms

Corrosion
GHS05

Signal Word

Danger

Hazard Statements

H314

Hazard Classifications

Eye Dam. 1 - Skin Corr. 1B

Storage Class Code

8A - Combustible corrosive hazardous materials

Certificates of Analysis (COA)

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Jeanine Montano et al.
Frontiers in microbiology, 11, 6-6 (2020-02-11)
Contamination of edible produce leaves with human bacterial pathogens has been associated with serious disease outbreaks and has become a major public health concern affecting all aspects of the market, from farmers to consumers. While pathogen populations residing on the
Sandra Meyer et al.
Nutrients, 12(4) (2020-04-08)
The present study tested the hypothesis that the liver lipid-lowering effect of insect meal (IM) is caused by its low methionine concentration. A total of fifty, male obese Zucker rats were randomly assigned to five groups of 10 rats each
Living with Glucose-6-Phosphate Dehydrogenase Deficiency
Devi A S, et al.
Journal of nursing science and practice., 6(1) (2016)
Rapid Elevation in CMPF May Act As a Tipping Point in Diabetes Development
Ying L, et al.
Cell Reports, 14(12), 2889-2900 (2016)
Redox Regulation of Cell Survival.
Dunyaporn T, et al.
Antioxidants & Redox Signaling, 10(8), 1343-1374 (2008)
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Protocols

Enzymatic Assay of Glucose-6-Phosphate Dehydrogenase (EC 1.1.1.49)

To measure glucose-6-phosphate dehydrogenase activity, beta-nicotinamide adenine dinucleotide phosphate is used in a spectrophotometric rate determination assay at 340 nm.

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