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M1254

Sigma-Aldrich

MOPS

≥99.5% (titration), crystalline powder

Synonym(s):

3-(N-Morpholino)propanesulfonic acid, 4-Morpholinepropanesulfonic acid

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About This Item

Empirical Formula (Hill Notation):
C7H15NO4S
CAS Number:
Molecular Weight:
209.26
Beilstein:
1106776
EC Number:
MDL number:
UNSPSC Code:
12161700
PubChem Substance ID:
NACRES:
NA.25

Pricing and availability is not currently available.

product name

MOPS, ≥99.5% (titration)

Quality Level

Assay

≥99.5% (titration)

form

crystalline powder

storage condition

dry at room temperature

technique(s)

affinity chromatography: suitable
electrophoresis: suitable

color

white

pH

2.5-4 (25 °C, 209 g/L)

useful pH range

6.5-7.9

pKa (25 °C)

7.2

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HNDG4MAG-36KHND3MAG-39KHNS2MAG-95K
manufacturer/tradename

Milliplex®

manufacturer/tradename

Milliplex®

manufacturer/tradename

Milliplex®

manufacturer/tradename

Milliplex®

assay range

accuracy: 107%
(DJ1), accuracy: 123%
(TG2), standard curve range: 15-60,000 pg/mL
(NSE), standard curve range: 244-1,000,000 pg/mL
(alpha-synuclein), standard curve range: 61-250,000 pg/mL
(TG2), accuracy: 108%
(a-Synuclein), standard curve range: 20-80,000 pg/mL
(UCHL1), standard curve range: 12-50,000 pg/mL
(GFAP), standard curve range: 61-250,000 pg/mL
(DJ1)

assay range

accuracy: 84-108%, standard curve range: 14-10,000 pg/mL
(S100B), standard curve range: 21-15,000 pg/mL
(sRAGE), standard curve range: 27-20,000 pg/mL
(Amyloid β42), standard curve range: 3-2,500 pg/mL
(Amyloid β40), standard curve range: 7-5,000 pg/mL
(GDNF)

assay range

accuracy: 69-92%
(Cerebrospinal fluid (CSF)), accuracy: 71-107%
(Serum), standard curve range: 0.03-25 ng/mL
(SOD2), standard curve range: 0.04-30 ng/mL
(Kallikrein-6), standard curve range: 0.04-30 ng/mL
(SOD1), standard curve range: 0.08-60 ng/mL
(Contactin-1), standard curve range: 0.14-100 ng/mL
(Osteopontin (OPN)), standard curve range: 1.37-1,000 ng/mL
(AGT), standard curve range: 4.12-3,000 ng/mL
(Fetuin A)

assay range

accuracy: 124%
(Neurogranin), accuracy: 84%
(FABP3), accuracy: 85%
(ApoE4), accuracy: 94%
(TREM2), accuracy: 97%
(Angiogenin), sensitivity: 11.0 pg/mL
(MinDC + 2SD; TREM2), sensitivity: 14.3 pg/mL
(MinDC + 2SD; FABP3), sensitivity: 162.8 pg/mL
(MinDC + 2SD; ApoE4), sensitivity: 19.9 pg/mL
(MinDC + 2SD; Neurogranin), sensitivity: 5.8 pg/mL
(MinDC + 2SD; Angiogenin), sensitivity: 6.1 pg/mL
(MinDC + 2SD; Ferritin), standard curve range: 15-50,000 pg/mL
(TREM2), standard curve range: 2-10,000 pg/mL
(Angiogenin), standard curve range: 24-100,000 pg/mL
(FABP3), standard curve range: 244-1,000,000 pg/mL
(ApoE4), standard curve range: 5-20,000 pg/mL
(Neurogranin), standard curve range: 6-25,000 pg/mL
(Ferritin), inter-assay cv: <10%
intra-assay cv: <5%
(Angiogenin), inter-assay cv: <10%
intra-assay cv: <5%
(FABP3), inter-assay cv: <10%
intra-assay cv: <5%
(TREM2), inter-assay cv: <15%
intra-assay cv: <10%
(Ferritin), inter-assay cv: <15%
intra-assay cv: <10%
(Neurogranin), inter-assay cv: <15%
intra-assay cv: <5%
(ApoE4)

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

-

technique(s)

multiplexing: suitable

technique(s)

multiplexing: suitable

technique(s)

multiplexing: suitable

technique(s)

multiplexing: suitable

shipped in

ambient

shipped in

wet ice

shipped in

wet ice

shipped in

-

General description

MOPS (3-(N-morpholino)propanesulfonic acid) also known as Propane Sultone buffer, is a versatile "Good′s buffer" extensively employed in biological research. It shares structural similarities with MES (2-(N-morpholino)ethanesulfonic acid), featuring a morpholine ring. In terms of physiological relevance, MOPS′ pKa of 7.2 closely aligns with physiological pH, making it suitable for studies requiring a near-neutral environment. Compared to MES with a pKa of 6.1, MOPS offers a more physiologically relevant buffering capacity.

MOPS serves various functions in diverse research domains. As a buffering agent, it can be used to stabilize pH in cell culture media, promoting optimal cell growth and function. Additionally, it may find application in protein purification in chromatography, stabilizes protein solutions, and acts as a reliable running buffer in various electrophoretic techniques, enabling efficient separation and analysis of biomolecules. MOPS also efficiently lyses cells for subsequent protein or nucleic acid extraction and stabilizes enzymes in solution, ensuring their activity for research purposes. It further proves valuable in fine-tuning the pH of growth media for optimal cell growth.

MOPS possesses additional advantages such as minimal interaction with metal ions especially with copper (Cu), nickel (Ni), manganese (Mn), zinc (Zn), cobalt (Co) ions, making it ideal for studies involving metal ions. It exhibits excellent water solubility, facilitating its use in various buffer formulations, and minimal lipid solubility, ensuring impermeability to membranes and minimizing interference with cellular processes. In summary, MOPS is a reliable buffer widely utilized across diverse biological disciplines, distinguished by its versatility, physiological relevance, minimal metal interaction, and ease of use, with its extensive applications in cell biology, protein studies, electrophoresis, and biochemical research.

Application

MOPS has been used as:

  • a cell culture additive component in lentiviral particle production[1]
  • as a buffering agent in microbial growth medium[2] and nuclei extraction buffer[3]
  • as a component of the MMSE-A buffer for isolating heart mitochondria[4]
  • as a component of the Synthetic Complete (SC) medium[5]
  • as a buffer in fluorescence quenching of NADH[6]

Features and Benefits

  • Ideal for Cell Biology and Biochemical research
  • Can be used as a Buffer component for Electrophoresis and Protein separation
  • Effective Buffering from pH 6.5-7.9 (25 °C) with a pKa of 7.2 (25 °C)
  • Highly soluble in water
  • Minimal metal ion binding

Other Notes

For additional information on our range of Biochemicals, please complete this form.

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

230.0 °F - closed cup

Flash Point(C)

110 °C - closed cup

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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(Un) suitability of the use of pH buffers in biological, biochemical and environmental studies and their interaction with metal ions-a review
Ferreira CMH, et al.
Royal Society of Chemistry Advances, 5(39), 30989-31003 (2015)
PROTOCOLS: Chromatin immunoprecipitation from Arabidopsis tissues
Yamaguchi N, et al.
The Arabidopsis Book / American Society of Plant Biologists, 12(3), e170-e170 (2014)
Survival to different acid challenges and outer membrane protein profiles of pathogenic Escherichia coli strains isolated from pozol, a Mexican typical maize fermented food
Sainz T, et al.
International Journal of Food Microbiology, 105(3), 357-367 (2005)
Detailed design and comparative analysis of protocols for optimized production of high-performance HIV-1-derived lentiviral particles
Mitta B, et al.
Metabolic engineering, 7(5-6), 426-436 (2005)
Interactions of biological buffers with macromolecules: The ubiquitous ?smart? polymer PNIPAM and the biological buffers MES, MOPS, and MOPSO
Taha M, et al.
Macromolecules, 44(21), 8575-8589 (2011)

Protocols

Simplify your SDS-PAGE gel preparation with our pre-mixed solutions and protocols to achieve precise protein separation.

Simplify your SDS-PAGE gel preparation with our pre-mixed solutions and protocols to achieve precise protein separation.

Simplify your SDS-PAGE gel preparation with our pre-mixed solutions and protocols to achieve precise protein separation.

Simplify your SDS-PAGE gel preparation with our pre-mixed solutions and protocols to achieve precise protein separation.

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