L6632
Lipoxidase from Glycine max (soybean)
Type V, ammonium sulfate suspension, 500,000-1,000,000 units/mg protein
Synonym(s):
Linoleate:oxygen oxidoreductase, Lipoxygenase
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About This Item
CAS Number:
Enzyme Commission number:
EC Number:
MDL number:
UNSPSC Code:
12352204
NACRES:
NA.54
Recommended Products
type
Type V
Quality Level
form
ammonium sulfate suspension
specific activity
500,000-1,000,000 units/mg protein
mol wt
94 kDa
storage temp.
2-8°C
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Application
Lipoxidase, or lipoxygenase, from Glycine max (soybean) has been used for the modification of low density lipoprotein, isolated from human plasma.
The soybean enzyme will use arachidonic acid as a substrate, with ~ 15% of the activity indicated using linoleic acid as the substrate; the product of arachidonic acid oxidation is 12- or 15-hydroperoxyarachidonic acid (12-HPETE or 15-HPETE).
Biochem/physiol Actions
Catalyzes the hydroperoxidation of lipids containing a cis,cis-1,4-pentadiene structure.
Unit Definition
One unit will cause an increase in A234 of 0.001 per min at pH 9.0 at 25 °C when linoleic acid is the substrate in 3.0 ml volume (1 cm light path). One A234 unit is equivalent to the oxidation of 0.12 μmole of linoleic acid.
Physical form
Suspension in 2.3 M (NH4)2SO4 solution, pH approx. 6.0
Preparation Note
Prepared by ion exchange chromatography and hydrophobic interaction chromatography.
Analysis Note
Protein determined by biuret.
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Resp. Sens. 1
Storage Class Code
11 - Combustible Solids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Certificates of Analysis (COA)
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Articles
Instructions for working with enzymes supplied as ammonium sulfate suspensions
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