L0636

Lectin from Triticum vulgaris (wheat)

suitable for cell culture, BioReagent, lyophilized powder

• Synonym(s): WGA, Wheat germ agglutinin
• UNSPSC Code: 12352202
• NACRES: NA.32

Properties

• sterility: aseptically processed
• Quality Level: 200
• product line: BioReagent
• form: lyophilized powder
• technique(s): cell culture | mammalian: suitable
• impurities: salt, essentially free
• storage temp.: 2-8°C

Description

General description

Lectin from Triticum vulgaris (wheat) is a dimeric glycoprotein of 36 kDa. It belongs to the family of chitin-binding proteins. Glycosylated precursor of lectin is synthesized in endoplasmic reticulum.

Application

Lectin from Triticum vulgaris (wheat) has been used:

• to conjugate with N-hydroxysuccinimide (NHS) dye
• to inhibit nuclear import
• to inhibit the nuclear pore of human cervical carcinoma HeLa and SiHa cells

Biochem/physiol Actions

Lectin from Triticum vulgaris (wheat) possessing four binding sites is specific for N-acetyl-d-glucosamine and N-acetyl-d-neuraminic acid (sialic acid) residues. Lectins also function as a fungicide. They also possess anti-nutritive effects.

WGA is not blood group specific but has an affinity for N-acetyl-β-D-glucosaminyl residues and N-acetyl-β-D-glucosamine oligomers. WGA contains no protein-bound carbohydrate.

Other Notes

Highly purified

Preparation Note

Conjugates are prepared from affinity purified lectin.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves