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I3275

Sigma-Aldrich

Interferon-γ from rat

≥97% (SDS-PAGE), recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture

Synonym(s):

IFN-γ

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About This Item

MDL number:
UNSPSC Code:
12352202
NACRES:
NA.32

biological source

rat

Quality Level

recombinant

expressed in E. coli

Assay

≥97% (SDS-PAGE)

form

lyophilized powder

mol wt

15.5 kDa

packaging

pkg of 0.1 mg

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

color

white

UniProt accession no.

storage temp.

−20°C

Gene Information

rat ... Ifng(25712)

Application

Interferon-γ from rat has been used to study the effect of lipoxin A4 and 17(R)-resolvin D1 on lipopolysaccharide and interferon-γ-mediated tumor necrosis factor release in rat astrocyte culture. In rats, it has been used in the treatment of INS-1 cell culture to study the conversion factors which change the frequency of β-cell replication or apoptosis to rates of replication or apoptosis.

Biochem/physiol Actions

Ifng (interferon-γ) plays an important role in innate and adaptive immune responses, and helps in defense against bacterial infections, dimorphic yeast and some viral infections. In rats, it is upregulated during ischemic injury in the embolic stroke. In cultured rat intestinal mucosa microvascular endothelial cells, ifng is responsible for inducing expression of interferon-α and interferon-β.

Features and Benefits

The biological activity is measured in an anti-viral assay using L-929 cells infected with EMC virus.

Physical form

Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline containing 50 μg of bovine serum albumin per 1 μg of cytokine.

Storage Class Code

13 - Non Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Meager, A. et al.
Lymphokines and Interferons, A Practical Approach, 129-129 (1987)
Sally Abdelmoaty et al.
PloS one, 8(9), e75543-e75543 (2013-10-03)
Lipoxins and resolvins have anti-inflammatory and pro-resolving actions and accumulating evidence indicates that these lipid mediators also attenuate pain-like behavior in a number of experimental models of inflammation and tissue injury-induced pain. The present study was undertaken to assess if
Zhen-Jie Wang et al.
iScience, 24(11), 103280-103280 (2021-11-13)
Nitric oxide (NO) is an important immune molecule that acts against extracellular and intracellular pathogens in most hosts. However, after the knockout of inducible nitric oxide synthase (iNOS -/-) in Sprague Dawley (SD) rats, these iNOS -/- rats were found
Hamid Reza Jafarinaveh et al.
Iranian journal of allergy, asthma, and immunology, 13(2), 125-130 (2013-12-18)
Increased levels of proinflammatory cytokines have been recorded after the onset of transient or permanent brain ischemia and are usually associated with exacerbation of ischemic injury. Embolic stroke model is more relevant to the pathophysiological situation in such patients, because
R Dijkema et al.
The EMBO journal, 4(3), 761-767 (1985-03-01)
The chromosomal immune interferon gene of the rat (IFN-gamma) was identified by screening a recombinant rat lambda phage library with a human IFN-gamma cDNA probe. In contrast to the genes of other rat IFNs, this rat IFN-gamma chromosomal gene contains

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