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H7782

Sigma-Aldrich

Hybridization solution

Synonym(s):

Hyb solution, Hybridization solution for in situ hybridization, Ready-to-use hybridization solution

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About This Item

EC Number:
200-842-0
UNSPSC Code:
12161503
NACRES:
NA.32

form

liquid

Quality Level

200

technique(s)

FISH: suitable

storage temp.

−20°C

Related Categories

General description

In situ hybridization assays detect a specific nucleic acid sequence of a gene at the cellular level. Hybridization solution is used to enhance hybrid formation between the DNA probe and the target mRNA on tissue sections. It may be used for reconstituting and/or diluting the probe for in situ hybridization experiments, or as a negative control (without probe).
Ready-to-use hybridization solution optimized for in situ hybridization (ISH) procedures.

Application

Hybridization solution has been used in:
  • fluorescent in situ hybridization (FISH) to perform probe annealing
  • in situ hybridization (ISH) to dilute probe for the detection of amastigote forms of Leishmania infantum
  • FISH analysis to hybridize probes

Pictograms

Health hazard
GHS08

Signal Word

Danger

Hazard Statements

H351,H360D,H373

Hazard Classifications

Carc. 2 - Repr. 1B - STOT RE 2 Oral

Target Organs

Blood

Storage Class Code

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

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Aurea V A da Silva et al.
Immunity, inflammation and disease, 8(2), 127-139 (2020-03-25)
In canine visceral leishmaniasis (CVL), lymphopenia, and the disorganization of lymphoid organs such as spleen and lymph nodes have been demonstrated. However, the involvement of thymus in CVL has not been evaluated so far. Herein, we investigated whether the thymus
Ying Lv et al.
Journal of the American Heart Association, 8(2), e010456-e010456 (2019-01-15)
Background Intrauterine growth retardation ( IUGR ) is related to pulmonary artery hypertension in adults, and mi croRNA -206 (miR-206) is proposed to affect the proliferation and apoptosis of pulmonary artery smooth muscle cells ( PASMC s) via post-transcriptional regulation.
Detection of characteristic heterochromatin distribution, highly amplified rRNA genes and presence of the human satellite III DNA motif in the scleractinian coral Echinophyllia aspera Ellis and Solander 1788
Taguchi T, et al.
Chromosome Analysis Protocols, 16, 33-38 (2013)
Melanie J Harriff et al.
Journal of bacteriology, 191(4), 1132-1142 (2008-12-09)
The Mycobacterium avium complex (MAC) is an important group of opportunistic pathogens for birds, cattle, swine, and immunosuppressed humans. Although invasion of epithelial cells lining the intestine is the chief point of entry for these organisms, little is known about

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