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H1009

Sigma-Aldrich

Hydrogen peroxide solution

30 % (w/w) in H2O, contains stabilizer

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About This Item

Empirical Formula (Hill Notation):
H2O2
CAS Number:
7722-84-1
Molecular Weight:
34.01
Beilstein:
3587191
MDL number:
MFCD00011333
UNSPSC Code:
12352304
PubChem Substance ID:
57654227
NACRES:
NA.77

vapor pressure

23.3 mmHg ( 30 °C)

Quality Level

200

Assay

29.0-32.0% (titration)

form

liquid

shelf life

~2 yr

reaction suitability

reagent type: oxidant

concentration

30 % (w/w) in H2O

technique(s)

cell based assay: suitable
immunohistochemistry: suitable

density

1.110 g/cm3

suitability

suitable for DNA precipitation

application(s)

general analytical
pharmaceutical

storage temp.

2-8°C

SMILES string

OO

InChI

1S/H2O2/c1-2/h1-2H

InChI key

MHAJPDPJQMAIIY-UHFFFAOYSA-N

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General description

Hydrogen peroxide (H2O2), a topical antiseptic, is synthesized in aerobic cells as a byproduct of aerobic respiration. It might also be produced as an output of enzymatic reactions in peroxisomes, mitochondria, or other cell compartments.

Application

Hydrogen peroxide solution has been used:
  • to treat nasal epithelial cells for H2O2 sensitivity assays
  • to treat the human immortal keratinocyte cell line (HaCaT) and the human melanoma cell line (GLL19) to induce senescence
  • to incubate brain sections for bromodeoxyuridine (BrdU)/NeuN staining
  • in cell proliferation assay to incubate neural stem cells (NE-4C) for in vitro experiments

Biochem/physiol Actions

Hydrogen peroxide (H2O2) is mainly used in wound cleaning. It can kill the pathogens through oxidation burst and by producing local oxygen. H2O2 functions as a signaling molecule or second messenger. It plays a key role in gene expression in numerous ways. H2O2 participates in modulating inflammation.

Features and Benefits

This compound is featured on the Phosphoprotein Phosphatases (Tyrosine) page of the Handbook of Receptor Classification and Signal Transduction. To browse other handbook pages, click here.

Other Notes

2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research
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Pictograms

Corrosion
GHS05

Signal Word

Danger

Hazard Statements

H318,H412

Hazard Classifications

Aquatic Chronic 3 - Eye Dam. 1

Storage Class Code

5.1B - Oxidizing hazardous materials

WGK

WGK 1

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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S V Lennon et al.
Cell proliferation, 24(2), 203-214 (1991-03-01)
Cell death may occur by either of two mechanisms: apoptosis or necrosis. Necrosis, the first type of cell death to be recognized, is an uncontrolled degenerative phenomenon invariably caused by noxious stimuli and is the result of irreversible failure of
Akitoshi Nakashima et al.
Scientific reports, 9(1), 11466-11466 (2019-08-09)
Pregnancy is a stress factor culminating into mild endoplasmic reticulum (ER) stress, which is necessary for placental development. However, excessive or chronic ER stress in pre-eclamptic placentas leads to placental dysfunction. The precise mechanisms through which excessive ER stress impacts
Shoichi Iriguchi et al.
Blood, 125(2), 370-382 (2014-10-29)
Although overexpression of T-bet, a master transcription factor in type-1 helper T lymphocytes, has been reported in several hematologic and immune diseases, its role in their pathogenesis is not fully understood. In the present study, we used transgenic model mice
Osamu Sakai et al.
Investigative ophthalmology & visual science, 56(1), 538-543 (2015-01-13)
The purpose of the present study was to investigate the role of glutathione peroxidase 4 (GPx4) in conjunctival epithelial cells. An immortalized human conjunctival epithelial cell line was used. Cells were transfected with catalase, GPx1, GPx4, SOD1, SOD2, or control
Maheswari Jayakannan et al.
Journal of experimental botany, 66(7), 1865-1875 (2015-01-24)
The role of endogenous salicylic acid (SA) signalling cascades in plant responses to salt and oxidative stresses is unclear. Arabidopsis SA signalling mutants, namely npr1-5 (non-expresser of pathogenesis related gene1), which lacks NPR1-dependent SA signalling, and nudt7 (nudix hydrolase7), which
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This solution is used as a substrate for detecting horseradish-peroxidase conjugates in Western blotting.

Protocols

Enzymatic Assay of Catalase (EC 1.11.1.6)

This procedure may be used for all Catalase products.

Enzymatic Assay of Peroxidase (EC 1.11.1.7) 2,2'-Azino-bis(3-Ethylbenzthiazoline-6-Sulfonic Acid) as a Substrate

To standardize a procedure for the assay of Peroxidase using 2,2'-Azino-bis(3-Ethylbenzthiazoline-6-Sulfonic Acid) as a substrate.

Enzymatic Assay of Peroxidase (EC 1.11.1.7)

This procedure is for the determination of Peroxidase enzymatic activity using Pyrogallol as the substrate.

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