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GS90

Sigma-Aldrich

GST T1-1, Recombinant Human

Synonym(s):

glutathione S-transferase theta 1

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About This Item

UNSPSC Code:
12352200
NACRES:
NA.26

biological source

human

Quality Level

recombinant

expressed in E. coli

Assay

95% (SDS-PAGE)

form

frozen liquid

specific activity

8.8 units/mg protein

mol wt

25 kDa

concentration

1.95 mg/mL

storage temp.

−70°C

Gene Information

human ... GSTT1(2952)

General description

Glutathione S-transferase theta 1 (GSTT1) and GSTT2/GSTT2B share 55% amino acid sequence identity and may play a role in human carcinogenesis. The GSTT1 gene is haplotype-specific and is absent from 38% of the population. Alternative splicing of this gene results in multiple transcript variants.GSTT1 is an enzyme that in humans is encoded by the GSTT1 gene. The GSTT1 gene contains five exons and four introns and spans 8092bp on genomic DNA. It is mapped to human chromosome 22q11.23. This gene codes for a 240 amino acids, which is mainly expressed in human liver. Based on their biochemical, immunologic, and structural properties, cytosolic and membrane-bound forms of glutathione S-transferase are encoded by two distinct supergene families. At present, eight distinct classes of the soluble cytoplasmic mammalian glutathione S-transferases have been identified: α, κ, μ, ω, π, σ, θ and ζ.
as assayed by the spectrophotometric determination of NADPH oxidation coupled to the glutathione peroxidase activity of GST T1-1 on cumene hydroperoxide (1.5 mM) in the presence of reduced glutathione (1 mM) in 100 mM NaPO4 (pH 7.0) at room temperature.

Biochem/physiol Actions

Glutathione S-transferase theta 1 (GSTT1) is an enzyme that in humans is encoded by the GSTT1 gene. Glutathione S-transferases (GSTs) are a family of enzymes that play an important role in detoxification by catalyzing the conjugation of many hydrophobic and electrophilic compounds with reduced glutathione. Mutation in the gene might lead to the development of lung, head and neck cancer. The GSTs are thought to function in xenobiotic metabolism and play a role in susceptibility to cancer, and other diseases.

Storage and Stability

The enzyme should be used by the end-user customer within 1 year of receipt.

Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

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Ender Coskunpinar et al.
Asian Pacific journal of cancer prevention : APJCP, 15(5), 2319-2321 (2014-04-11)
Our aim was to determine GSTT1 expression levels in left colon tumors and paired normal tissue in order to identify specific alterations in GSTT1 mRNA levels. Alterations in GSTT1 expression in twenty-four left- sided colon tumors and paired cancer free
Bacterial glutathione S-transferases: what are they good for?
Vuilleumier, S, et al.
Journal of Bacteriology, 179, 1431-1431 (1997)
GSTT1 null genotype contributes to lung cancer risk in asian populations: a meta-analysis of 23 studies
Yang, X, et al.
PLoS ONE, 8 (2013)
Association of GSTM1 and GSTT1 gene deletions with risk of head and neck cancer in Pakistan: a case control study
Nosheen, M, et al.
Asian Pacific Journal of Cancer Prevention, 11, 881-885 (2010)
Alfredo Santovito et al.
Journal of biochemical and molecular toxicology, 29(5), 234-239 (2015-02-04)
Occupational exposure to anaesthetic gases is one of the major hazards to healthcare personnel. We evaluated the cytogenetic effects of chronic exposure to low concentrations of anaesthetic gases in operating theatres. The study included 21 anesthetists and 21 control subjects

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