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GE17-0404-03

HiTrap® Protein G High Performance

Cytiva 17-0404-03, pack of 2 × 1 mL

Synonym(s):

HiTrap Protein G, Protein G affinity column

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About This Item

UNSPSC Code:
23151816
NACRES:
NA.56

ligand

recombinant protein G lacking albumin-binding region

form

prepacked column

crosslinking

cross-linked

packaging

pack of 2 × 1 mL

manufacturer/tradename

Cytiva 17-0404-03

storage condition

(20% Ehtanol)
 (20% Ethanol)

parameter

42 psi

technique(s)

liquid chromatography (LC): suitable

bed size

7 mm × 25 mm

bed volume

1 mL

column I.D.

7 mm

size

matrix

(cross-linked agarose, spherical)

matrix active group

Protein G

avg. part. size

34 μm

cleaning

2-9

working range

3-9

capacity

>1 mg binding capacity (lgG/column)
 25 mg binding capacity(human IgG/column)

separation technique

affinity

storage temp.

2-8°C

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General description

HiTrap® Protein G HP columns are prepacked with Protein G Sepharose™ High Performance for fast, easy separations of IgG from different species and subclasses.
The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.
pH below 3 is sometimes required to elute strongly bound IgG species. However, protein ligands may hydrolyze at very low pH.
pH below 3 is sometimes required to elute strongly bound IgG species. However, protein ligands may hydrolyze at very low pH.

Features and Benefits

  • High binding capacity of 25 mg IgG/mL medium and fast binding kinetics.
  • 34 μm bead size for high performance, high-resolution purifications.
  • Binds broad range of different species and subclasses of IgG.
  • Simple and proven method giving reproducible results.
  • Designed for use with syringe, peristalic pump, or chromatography system.

Storage and Stability

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Legal Information

HiTrap is a registered trademark of Cytiva
Sepharose is a trademark of Cytiva

related product

Product No.
Description
Pricing

Pictograms

Flame
GHS02

Signal Word

Warning

Hazard Statements

H226

Storage Class Code

3 - Flammable liquids

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Nimitha R Mathew et al.
Cell reports, 35(12), 109286-109286 (2021-06-24)
B cell responses are critical for antiviral immunity. However, a comprehensive picture of antigen-specific B cell differentiation, clonal proliferation, and dynamics in different organs after infection is lacking. Here, by combining single-cell RNA and B cell receptor (BCR) sequencing of

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Protocols

Two-step Purification of Mouse Monoclonal IgG1 Using HiTrap® Protein G HP for the Capture Step

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