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G6019

Sigma-Aldrich

Glyceraldehyde-3-phosphate Dehydrogenase from human erythrocytes

lyophilized powder, 50-150 units/mg protein

Synonym(s):

D-Glyceraldehyde 3-phosphate:NAD+ oxidoreductase (phosphorylating), GAPDH

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About This Item

CAS Number:
9001-50-7
Enzyme Commission number:
1.2.1.12 (BRENDA, IUBMB)
MDL number:
MFCD00131203
UNSPSC Code:
12352204
NACRES:
NA.54

form

lyophilized powder

Quality Level

200

specific activity

50-150 units/mg protein

composition

Protein, 40-70% biuret

UniProt accession no.

P04406

foreign activity

3-Phosphoglyceric phosphokinase ≤2%

storage temp.

−20°C

Gene Information

human ... GAPDH(2597)

Biochem/physiol Actions

Glyceraldehyde-3-phosphate dehydrogenase catalyzes the conversion of glyceraldehyde-3-phosphate to 1,3-bisphosphoglycerate as part of glycolysis. It has also been shown to have roles in initiation of apoptosis, transcription activation and the shuttling of ER to Golgi vesicles<<<18,New>>>.

Unit Definition

One unit will reduce 1.0 μmole of 3-phosphoglycerate to D-glyceraldehyde 3-phosphate per min in a coupled system with 3-phosphoglyceric phosphokinase at pH 7.6 at 25 °C.

Physical form

Lyophilized powder containing sodium citrate buffer salts

inhibitor

Product No.
Description
Pricing

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Bing Fang et al.
Biochimie, 95(2), 251-257 (2012-09-27)
siRNA is promising in anti-tumor therapy. The main challenge is lack of tumor-specific intracellular delivery. In this study, a 6 amino acids peptide (A1) with high affinity for vascular endothelial growth factor receptor-1 (VEGFR1) was conjugated with a cell penetrating
Yuki Ikeda et al.
Archives of biochemistry and biophysics, 528(2), 141-147 (2012-09-25)
Cyclooxygenase (COX)-2 is an inducible inflammatory protein whose expression is partially regulated at the post-transcriptional level. We investigated whether glyceraldehyde-3-phosphate dehydrogenase (GAPDH) binds to the AU-rich element (ARE) of COX-2 mRNA for its degradation. Knockdown of GAPDH in hepa1c1c7 cells
Tina C Albershardt et al.
Journal of immunological methods, 384(1-2), 196-199 (2012-08-14)
Normalization to a reference gene is the method of choice for quantitative PCR analysis. The stability of reference genes is critical for accurate gene expression analysis, as significant variations in reference gene expression can alter experimental results and conclusions. In
Irina A Sevostyanova et al.
Biochemical and biophysical research communications, 427(3), 649-653 (2012-10-03)
Sperm-specific glycolytic enzyme glyceraldehyde-3-phosphate dehydrogenase (GAPDS) is normally expressed only in sperms, but not in somatic tissues. Analysis of the expression of GAPDS mRNA in different cancer cell lines shows that the content of GAPDS mRNA is enhanced in some
Chiaki Inadomi et al.
Biochemical and biophysical research communications, 425(3), 656-661 (2012-08-01)
There is increasing evidence demonstrating that glutaredoxin 1 (GRX1), a cytosolic enzyme responsible for the catalysis of protein deglutathionylation, plays distinct roles in inflammation and apoptosis by inducing changes in the cellular redox system. In this study, we investigated whether
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