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F3378

Sigma-Aldrich

Fast Blue BB Salt hemi(zinc chloride) salt

Dye content ≥80 %, Powder

Synonym(s):

4-Amino-2,5-diethoxybenzanilide diazotated zinc double salt, 4-Benzoylamino-2,5-diethoxybenzenediazonium chloride hemi(zinc chloride) salt

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About This Item

Linear Formula:
C17H18N3O3Cl · 1/2 ZnCl2
CAS Number:
Molecular Weight:
415.94
Colour Index Number:
37175
Beilstein:
1845288
EC Number:
MDL number:
UNSPSC Code:
12171500
PubChem Substance ID:
NACRES:
NA.47

product name

Fast Blue BB Salt hemi(zinc chloride) salt, Dye content ≥80 %

form

powder

composition

Dye content, ≥80%

technique(s)

microbe id | staining: suitable

mp

157 °C

solubility

H2O: 10 mg/mL

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

−20°C

SMILES string

[Cl-].[Cl-].Cl[Zn]Cl.CCOc1cc([N+]#N)c(OCC)cc1NC(=O)c2ccccc2.CCOc3cc([N+]#N)c(OCC)cc3NC(=O)c4ccccc4

InChI

1S/2C17H17N3O3.4ClH.Zn/c2*1-3-22-15-11-14(20-18)16(23-4-2)10-13(15)19-17(21)12-8-6-5-7-9-12;;;;;/h2*5-11H,3-4H2,1-2H3;4*1H;/q;;;;;;+2/p-2

InChI key

CMFRFQODFZBKTI-UHFFFAOYSA-L

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General description

Fast Blue BB Salt hemi(zinc chloride) salt, also known as Cl Azoic diazo 20 (Cl 37175) is a diazonium salt that possesses a lipophilic amine lipophilic with an aromatic system of moderate size. It is commercially available as the zinc chloride double salt.

Application

Fast Blue BB Salt hemi(zinc chloride) salt has been used for alkaline phosphatase staining in cells.
Fast blue BB salt is suitable for the staining of enterochromaffin. Its applications are seen in enzyme histochemistry by Gomori and are suitable for the histochemical demonstration of beta-glucuronidase activities, and various peptidases and proteases. It is also used in enzyme histochemistry including the demonstration of serine esterase in blood smears and histological sections. Fast blue BB salt plays a vital role as a routine stain to demonstrate alkaline phosphatase labeled antibodies.

Preparation Note

Purified

Pictograms

Exclamation mark

Signal Word

Warning

Hazard Statements

Precautionary Statements

Hazard Classifications

Acute Tox. 4 Oral

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Enzyme-histochemical method for identification of lymphatic capillaries.
Kato S
Lymphology, 24, 125-125 (1991)
Behavior of ?-tricalcium phosphate granules composed of rod-shaped particles in the rat tibia.
Okumura T
J. Ceram. Soc. Jpn., 119, 101-104 (2011)
Marjorie B Medina
Journal of agricultural and food chemistry, 59(5), 1565-1571 (2011-02-12)
A new method for the detection of phenolics in food systems was developed. This method is based on interactions of phenolics with Fast Blue BB diazonium salt in alkali pH, forming azo complexes, with the absorbance measured at 420 nm
L Wagner et al.
Journal of immunological methods, 142(2), 147-155 (1991-09-13)
A trypsin-like serine esterase (SE) is known to be present in cultured cells with cytolytic potential. The distribution pattern of this enzyme in haematological cells and body tissues has been assessed using a method which permits rapid identification of individual
B Algermissen et al.
Experimental dermatology, 3(6), 290-297 (1994-12-01)
In order to gain insights into the dynamics of mast cell subpopulations in normal and diseased skin, a novel enzyme-histochemical double and triple staining method was employed that allowed the detection of metachromasia (toluidine blue) and the mast cell proteases

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