C0794

Chitosanase from Streptomyces sp.

buffered aqueous glycerol solution, ≥15 units/mg protein (E^1%)

• Synonym(s): Chitosan N-acetylglucosaminohydrolase
• CAS Number: 51570-20-8
• Enzyme Commission number: 3.2.1.132 (BRENDA, IUBMB)
• MDL number: MFCD00211797
• UNSPSC Code: 12352204
• NACRES: NA.54

Properties

• form: buffered aqueous glycerol solution
• Quality Level: 200
• specific activity: ≥15 units/mg protein (E^1%)
• concentration: ≥0.5 mg/mL
• solubility: 0.1 M acetate buffer, pH 5.5: soluble
• storage temp.: −20°C

Description

Application

Chitosanase from Sigma has been used for polarized light-stimulated, enzymatic hydrolysis of chitin and chitosan.

Biochem/physiol Actions

Chitosanase catalyzes the endohydrolysis of β-1,4-linkages between D-glucosamine (GlcN-GlcN) residues in chitosan. The enzyme from Streptomyces has been reported to also hydrolyze the GlcNAc-GlcN linkage in partially acetylated chitosan.

Unit Definition

One unit will liberate 1.0 μmole of reducing sugar (measured as D-glucosamine equivalents) from chitosan per minute at pH 5.5 at 37 °C.

Physical form

Solution in 50% glycerol containing 50 mM sodium acetate

Preparation Note

Soluble in 10mL of 0.1 M Acetate buffer at pH 5.5

Other Notes

View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable