A8580
Anti-Glutathione-S-Transferase (GST)–Agarose antibody produced in rabbit
IgG fraction of antiserum, PBS suspension
Synonym(s):
Anti-Glutathione-S-Transferase, Anti-GST
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About This Item
UNSPSC Code:
12352203
NACRES:
NA.46
Recommended Products
biological source
rabbit
Quality Level
conjugate
agarose conjugate
antibody form
IgG fraction of antiserum
antibody product type
primary antibodies
clone
polyclonal
form
PBS suspension
mol wt
antigen 27.5 kDa
should not react with
porcine, bovine, human, rabbit, rat
technique(s)
ELISA: suitable
immunoprecipitation (IP): suitable
shipped in
wet ice
storage temp.
2-8°C
target post-translational modification
unmodified
Gene Information
human ... GSTP1(2950)
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General description
Glutathione-S-transferases (GSTs) are dimeric proteins, which belongs to phase II detoxification enzymes family. The highly polymorphic human cytosolic GSTs are divided into six classes, such as, α, μ, ω, π, θ, and ζ.
The antibody is specific for native as well as denatured-reduced forms of glutathione-S-transferase from Schistosoma japonicum. Anti-GST may be used in various immunoassays to identify the expression of GST fusion proteins.
Immunogen
recombinant GST from Schistosoma japonicum expressed in E. coli.
Application
Immunoprecipitation was performed on NIH 3T3 cells transiently transfected with GST fusion proteins. IP was performed using agarose-linked rabbit anti-GST IgG with mixing for 2 hours at 4 degrees.
Rabbit Anti-Glutathione-S-Transferase (GST)-Agarose antibody can be used for ELISA, immunoaffinity purification and immunoprecipitation assays.
Biochem/physiol Actions
Glutathione-S-transferases (GSTs) can catalyse the conjugation of glutathione (GSH) to various endogenous and exogenous electrophilic compounds. The π and μ classes of GSTs controls the mitogen-activated protein (MAP) kinase pathway. It can also serve as transport proteins.
Physical form
Suspension in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide
Preparation Note
Prepared by coupling cyanogen bromide-activated agarose at 7-8 mg antibody per mL resin volume.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
10 - Combustible liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
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