A5795
Anti-Rat IgG (whole molecule)−Peroxidase antibody produced in rabbit
affinity isolated antibody, buffered aqueous solution
Synonym(s):
HRP Rabbit Anti-Rat IgG (whole molecule)
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About This Item
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biological source
rabbit
Quality Level
conjugate
peroxidase conjugate
antibody form
affinity isolated antibody
antibody product type
secondary antibodies
clone
polyclonal
form
buffered aqueous solution
species reactivity
rat
should not react with
human
technique(s)
direct ELISA: 1:50,000
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:750
western blot (chemiluminescent): 1:80,000-1:160,000 using rat PC12 cell extract
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
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General description
An immunoglobulin has two heavy chain and two light chain connected by disulfide bond. It mainly helps in immune defense. It is a glycoprotein. IgG is a major class of immunoglobulin. Rat has five immunoglobulin classes- IgM, IgG, IgA, IgD and IgGE. IgG has four subclasses- IgG1, IgG2a, IgG2b and IgG2c.
IgGs are glycoprotein antibodies that modulate several immune responses. Rat IgGs against target proteins are often used as primary antibodies in various research applications. Thus, secondary anti-rat IgGs conjugated to a detectable substrate are useful tools for the analysis of target proteins.
Anti-Rat IgG (whole molecule)-Peroxidase antibody binds all rat Igs and does not react with human IgG.
Anti-Rat IgG (whole molecule)-Peroxidase antibody binds all rat Igs and does not react with human IgG.
Immunogen
purified rat IgG
Application
Anti-Rat IgG (whole molecule)-Peroxidase antibody has been used as a secondary antibody in immunohistochemical applications at a dilution of 1:1500. The product has also been used for western blot analysis at 1:20,000. Furthermore, the antibody can be used for direct ELISA at 1:50,000.
Anti-Rat IgG (whole molecule)?Peroxidase antibody produced in rabbit has been used in immunohistochemistry and western blot analysis.
HA-tagged proteins over expressed in S. cerevisiae were detected by western blot using HRP-conjugated rabbit anti-rat IgG as the secondary antibody.
Biochem/physiol Actions
Immunoglobulin (IgG) helps in opsonization, complement fixation and antibody dependent cell mediated cytotoxicity. IgG participates in hypersensitivity type II and type III.
Physical form
Solution in 0.01 M phosphate buffered saline pH 7.4, containing 0.05% MIT
Preparation Note
Adsorbed with human IgG to reduce background staining with human samples.
Prepared by two-step glutaraldehyde method.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Signal Word
Warning
Hazard Statements
Precautionary Statements
Hazard Classifications
Skin Sens. 1
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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