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Sigma-Aldrich

5-Cyano-2,3-di-(p-tolyl)tetrazolium chloride

≥85% (HPLC)

Synonym(s):

5-Cyano-2,3-bis(4-methylphenyl)-2H-tetrazolium chloride, CTC

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About This Item

Empirical Formula (Hill Notation):
C16H14ClN5
CAS Number:
90217-02-0
Molecular Weight:
311.77
Beilstein:
8954451
MDL number:
MFCD00467521
UNSPSC Code:
12171500
PubChem Substance ID:
57653613
NACRES:
NA.32

Quality Level

100

Assay

≥85% (HPLC)

form

powder

storage temp.

2-8°C

SMILES string

[Cl-].Cc1ccc(cc1)-n2nc(n[n+]2-c3ccc(C)cc3)C#N

InChI

1S/C16H14N5.ClH/c1-12-3-7-14(8-4-12)20-18-16(11-17)19-21(20)15-9-5-13(2)6-10-15;/h3-10H,1-2H3;1H/q+1;/p-1

InChI key

SSJZAXOTLCJNLF-UHFFFAOYSA-M

General description

5-cyano-2,3-di-(p-tolyl)-tetrazolium chloride is a Redox Sensitive tetrazolium salt producing a fluorescent formazan (only in solid state) upon reduction; It indicates the respiratory activity in cells.

Application

5-cyano-2,3-di(p-tolyl) tetrazolium chloride (CTC)-reduction test is used for live/dead analysis. 5-cyano-2,3-di-(p-tolyl)-tetrazolium chloride has been employed in histochemistry and for determining cell viability, for eukaryotic cell viability assays and bacterial viability experiments, 5-cyano-2,3-di-(p-tolyl)-tetrazolium chloride (CTC) is the most often employed compound.

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Pricing

Pictograms

Exclamation mark
GHS07

Signal Word

Warning

Hazard Statements

H315,H319,H335

Hazard Classifications

Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3

Target Organs

Respiratory system

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Juan C Stockert et al.
Acta histochemica, 120(3), 159-167 (2018-03-03)
For many years various tetrazolium salts and their formazan products have been employed in histochemistry and for assessing cell viability. For the latter application, the most widely used are 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT), and 5-cyano-2,3-di-(p-tolyl)-tetrazolium chloride (CTC) for viability assays of
Rodriguez, G.G., et al.
Applied and Environmental Microbiology, 58, 180-180 (1992)
Severin, E., et al.
Analytica Chimica Acta, 170, 341-341 (1985)
Yuta Morishige et al.
Biological & pharmaceutical bulletin, 37(10), 1617-1625 (2014-08-12)
An environmental isolate of Salmonella enterica serovar Enteritidis (SE) clone, SE Cl#15-1, loses its culturability during 72-h culture in M9 minimal medium containing 0.8% glucose, a concentration twice higher than that in normal M9 medium, whereas the bacterium retains its

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