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product name
P4E6 cell line,
biological source
human prostate
Quality Level
growth mode
Not specified
karyotype
Not specified
morphology
Epithelial
products
Stains positive for pancytokeratin, cytokeratin 8, vimentin (weak) and chromogranin A. Prostate markers expressed include prostate specific antigen (PSA) prostate specific membrane antigen (PSMA), negative for expression of androgen receptor (AR).
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cancer
shipped in
dry ice
storage temp.
−196°C
Cell Line Origin
Prostate cancer well differentiated, early stage
DNA Profile
STR-PCR Data:
Amelogenin: X
CSF1PO: 12
D13S317: 11
D16S539: 11
D5S818: 12
D7S820: 7,8
THO1: 7,9.3
TPOX: 8
vWA: 17,19
Amelogenin: X
CSF1PO: 12
D13S317: 11
D16S539: 11
D5S818: 12
D7S820: 7,8
THO1: 7,9.3
TPOX: 8
vWA: 17,19
Culture Medium
Stemline Keratinocyte Medium II (Sigma S0196) + Stemline Keratinocyte Growth Supplement (Sigma S9945) + 2 mM Glutamine + 2% Fetal Bovine Serum (FBS). Addition of 2% serum was found to increase cell viability.
Subculture Routine
Split sub-confluent cultures (70-80%) 1:4 to 1:10 seeding at approximately 3 x 104 cells/cm2 using 0.05% trypsin or trypsin/EDTA; 5% CO2; 37 °C. It is important to inactivate the trypsin with an equal volume of trypsin inhibitor (the culture medium contains too low a serum content to inactivate the trypsin). To remove all traces of trypsin and trypsin inhibitor pellet the cells by centrifugation and resuspend the cells in fresh medium. Media change every second day.
Other Notes
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
Disclaimer
RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.
This cell line has special release conditions: Commercial organisations are required to complete the ′Cell Line Release Authorisation for Research Use in Commercial Organisations′ release conditions form. Additionally, any recipient wanting to use the cell line in the field of vaccines for the treatment or prevention of prostate cancer should first obtain permission from GemVax & KAEL Co., Ltd. Contact : Dr. Eujin Hyun, Head of R&D Department (eujinhyun@kaelgemvax.com).
Storage Class Code
10 - Combustible liquids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Find documentation for the products that you have recently purchased in the Document Library.
N J Maitland et al.
Radiation research, 155(1 Pt 2), 133-142 (2000-12-20)
In Vitro Models to Study Cellular Differentiation and Function in Human Prostate Cancers. To augment the currently available models of human prostate cancer in vitro, we have established extended life-span epithelial cultures from biopsies of well-differentiated prostate cancers. The genetic
Shona H Lang et al.
The Prostate, 52(4), 253-263 (2002-09-05)
The metastatic potential of a series of prostate cell lines was analysed by measuring motility and invasiveness, and further correlated to the expression of epithelial differentiation markers. Invasion and motility were measured using in vitro assays. Immunohistochemistry of cell lines
Immortalization of human prostate cells with the human papillomavirus type 16 E6 gene.
Norman J Maitland et al.
Methods in molecular medicine, 88, 275-285 (2003-11-25)
Shona H Lang et al.
In vitro cellular & developmental biology. Animal, 42(8-9), 273-280 (2006-12-14)
Three-dimensional epithelial culture models are widely used to emulate a more physiologically relevant microenvironment for the study of genes and signaling pathways. Prostate epithelial cells can grow into solid cell masses or acinus-like spheroids in Matrigel. To test if the
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