P1379
TWEEN® 20
viscous liquid
Synonym(s):
Polyethylene glycol sorbitan monolaurate, Polyoxyethylenesorbitan monolaurate
About This Item
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description
non-ionic
Quality Level
Assay
>40% (GC)
form
viscous liquid
mol wt
~1228
composition
lauric acid, ≥40% (balance primarily myristic, palmitic, and stearic acids)
concentration
≥40.0% (GC)
technique(s)
DNA extraction: suitable
RNA extraction: suitable
cell culture | mammalian: suitable
protein purification: suitable
impurities
≤3.0% water
refractive index
n20/D 1.468 (lit.)
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General description
Surfactants, possessing both hydrophilic and hydrophobic components, fall into two main categories: ionic, which ionize in water (anionic, cationic, or ampholytic), and nonionic, which do not ionize. The discussed product is a nonionic surfactant with diverse applications such as emulsification, dispersion, solubilization, and detergent use. Additionally, it can serve as a solubilizer for membrane proteins.
Application
TWEEN 20 has been used as a blocking agent for membrane based immunoassays at a typical concentration of 0.05%. TWEEN 20 can be used for lysing mammalian cells at a concentration of 0.005 to 0.5%.
Features and Benefits
- Highly versatile surfactant for your life science and biochemical research
- Excellent solubilising, dispersing and emulsifying agent
- Suitable for DNA & RNA extraction, mammalian cell culture, and protein purification
Packaging
Each kit contains 3 x 100ML samples, each sample from a uniquely manufactured lot.
Preparation Note
Other Notes
Legal Information
also commonly purchased with this product
comparable product
recommended
Storage Class Code
10 - Combustible liquids
WGK
WGK 3
Flash Point(F)
527.0 °F - Pensky-Martens closed cup
Flash Point(C)
275 °C - Pensky-Martens closed cup
Personal Protective Equipment
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Protocols
This page shows how to solubilize membrane proteins with products from Cytiva.
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