E2028

Ethidium bromide monoazide

≥95% purity (HPLC), solid

• Synonym(s): 3-Amino-8-azido-5-ethyl-6-phenylphenanthridinium bromide, Ethidium monoazide bromide
• Empirical Formula (Hill Notation): C₂₁H₁₈BrN₅
• CAS Number: 58880-05-0
• Molecular Weight: 420.31
• MDL number: MFCD01708677
• UNSPSC Code: 12171500
• PubChem Substance ID: 329799025
• NACRES: NA.47

Properties

• product name: Ethidium bromide monoazide, ≥95% (HPLC), solid
• Quality Level: 100
• Assay: ≥95% (HPLC)
• form: solid
• technique(s): titration: suitable
• color: orange
• solubility: DMF: soluble; ethanol: soluble
• ε (extinction coefficient): ≥5200 at 459-465 nm
• application(s): diagnostic assay manufacturing; hematology; histology
• storage temp.: −20°C
• SMILES string: [Br-].CC[n+]1c(-c2ccccc2)c3cc(ccc3c4ccc(N)cc14)N=[N+]=[N-]
• InChI: 1S/C21H17N5.BrH/c1-2-26-20-12-15(22)8-10-18(20)17-11-9-16(24-25-23)13-19(17)21(26)14-6-4-3-5-7-14;/h3-13,22H,2H2,1H3;1H
• InChI key: GHUXAYLZEGLXDA-UHFFFAOYSA-N

Description

Biochem/physiol Actions

Photoactive stain covalently binds to nucleic acids in solution and in cells with damaged membranes.

Used to footprint drug binding sites on DNA, detect non-viable cells, identify ethidium binding sites on DNA and tRNA, and selectively inactivate gene expression.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves