85463

Simmons Citrate Agar

NutriSelect^® Basic, suitable for microbiology

• Synonym(s): Citrate Utilization Test
• UNSPSC Code: 41171606
• NACRES: NA.74

Properties

• sterility: non-sterile
• Quality Level: 100
• product line: BioChemika
• form: powder
• shelf life: limited shelf life, expiry date on the label
• composition: agar, 15 g/L ; ammonium dihydrogen phosphate, 0.2 g/L ; bromothymol blue, 0.08 g/L ; disodium ammonium phosphate, 0.8 g/L ; magnesium sulfate heptahydrate, 0.2 g/L ; sodium chloride, 5 g/L ; trisodium citrate, 2 g/L
• manufacturer/tradename: NutriSelect^® Basic
• technique(s): microbe id | utilization test: suitable; microbiological culture: suitable
• final pH: ~7.0
• application(s): agriculture; clinical testing; environmental; food and beverages; water monitoring; microbiology
• suitability: nonselective and differential for Escherichia coli; nonselective and differential for Salmonella spp.; nonselective and differential for coliforms; nonselective and differential for molds (General Media); nonselective and differential for yeasts (General Media)

Description

General description

Simmons Citrate Agar uses citric acid as the carbon source. It is used to differentiate Salmonella groups from each other. It does not allow the growth of Shigella or coliforms.

Application

Simmons Citrate Agar can be used for differentiating between fecal coli and members of the aerogenes group based on citrate utilization. It can also be used for the identification of certain fungi and fungi imperfecti. The agar has been used to perform a citrate test.

Preparation Note

Suspend 23.3 g in 1 litre distilled water and heat to boiling to dissolve. Distribute in tubes or flasks and sterilize by autoclaving at 121°C for 15 minutes. Allow tubes to cool as slants.

Other Notes

Selective recovery of Klebsiella pneumoniae and Klebsiella oxytoca from human feces

Footnote

We offer two media types: the superior granulated GranuCult^® and the cost-efficient powdered NutriSelect^® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany

NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 2
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves