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43925

Sigma-Aldrich

Nigrosin Stain solution

for microscopy

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About This Item

CAS Number:
MDL number:
UNSPSC Code:
12171500
NACRES:
MA.02

grade

for microscopy

Quality Level

form

liquid

shelf life

limited shelf life, expiry date on the label

technique(s)

microbe id | staining: suitable

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

suitability

Bacillus spp.
Corynebacterium spp.

General description

Negative staining method permits visualization of the usually transparent and unstainable capsule of many organisms, most importantly Cryptococcus neoformans.

Components

Composition:
Nigrosin 100 g/l, Formalin 5 ml/l in water

Pictograms

Health hazardExclamation mark

Signal Word

Danger

Hazard Statements

Hazard Classifications

Carc. 1B - Skin Sens. 1

Storage Class Code

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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G E Freneau et al.
Animal reproduction science, 118(2-4), 176-181 (2009-09-30)
The objectives of this study were to compare two different methods of evaluating bull sperm morphology, bright-field (BF) microscopy of eosin-nigrosin (EN) stained dry-mount semen smears and differential interference phase contrast (DIC) microscopy of wet-mount semen 'fixed' in isotonic formal
J Moshonov et al.
Endodontics & dental traumatology, 11(5), 220-224 (1995-10-01)
The purpose of this study was to assess the efficacy of Nd:YAG laser irradiation in disinfecting the root canal system. Seventy-five teeth were uniformly instrumented and sterilized, and their root canals were infected for 60 min with an overnight culture
K Matsuda et al.
Journal of clinical laboratory analysis, 15(4), 171-174 (2001-07-04)
We designed a semiquantitative analysis of urinary low protein levels using silver dot blot assay. In this method, 3 microl of urine are blotted to one dot onto a cellulose acetate membrane, which is stained by a colloidal silver staining
D Kutter et al.
Clinica chimica acta; international journal of clinical chemistry, 258(2), 231-239 (1997-02-17)
Nigrosin binds to proteins yielding a dark blue complex. This reaction is used for detecting low protein concentrations in urine by applying spots of urine to cellulose acetate strips which are coloured by nigrosin. The intensity of coloured spots remaining
Jie Guo et al.
Environmental science & technology, 42(2), 624-628 (2008-02-21)
Nonmetals reclaimed from waste phenolic cellulose paper printed circuit boards (PCBs) are used to replace wood flour in the production of phenolic molding compound (PMC). The results indicate that filling of nonmetals in PMC improves the charpy notched impact strength

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